Transcript profiling of Zea mays roots reveals gene responses to phosphate deficiency at the plant- and species-specific levels
Transcript profiling of Zea mays roots reveals gene responses to phosphate deficiency at the plant- and species-specific levels
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DOI：
10.1093/jxb/ern115
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发表时间：
2008-06-01
影响因子：
6.9
通讯作者：
Herrera-Estrella, Luis
中科院分区：
文献类型：
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作者：
Calderon-Vazquez, Carlos;Ibarra-Laclette, Enrique;Herrera-Estrella, Luis
玉米（Zea mays）是世界上种植最广泛的作物；然而，在许多地区，特别是在发展中国家的酸性和碱性土壤中，它通常受到磷酸盐（Pi）缺乏的影响。为了应对缺磷，植物进化出大量的发育和生化适应；然而，对于玉米来说，这些反应的潜在分子基础仍然未知。在这项工作中，我们评估了玉米根系在Pi剥夺开始后1、3、6和10 d对Pi饥饿的转录反应。共发现1179个pi应答基因，其中上调和下调基因分别为820个和363个，上调和下调幅度分别为2倍或2倍以上。pi响应基因被发现参与各种代谢、信号转导和发育基因网络。发现了大量的转录因子，这些转录因子可能是作物育种的潜在目标。此外，基因表达谱与特定代谢物的变化也存在相关性。结果表明，玉米对缺磷的几种双子叶植物反应是保守的，但一些遗传反应似乎更具特异性，缺磷导致玉米根系内部缺磷循环的转变。最终，这项工作为经济上重要的谷物提供了一个更全面的pi响应模型，并为生产pi特异性玉米微阵列建立了框架，以研究pi高效和pi低效玉米基因型之间全局基因表达的变化。
Maize (Zea mays) is the most widely cultivated crop around the world; however, it is commonly affected by phosphate (Pi) deficiency in many regions, particularly in acid and alkaline soils of developing countries. To cope with Pi deficiency, plants have evolved a large number of developmental and biochemical adaptations; however, for maize, the underlying molecular basis of these responses is still unknown. In this work, the transcriptional response of maize roots to Pi starvation at 1, 3, 6, and 10 d after the onset of Pi deprivation was assessed. The investigation revealed a total of 1179 Pi-responsive genes, of which 820 and 363 genes were found to be either up- or down-regulated, respectively, by 2-fold or more. Pi-responsive genes were found to be involved in various metabolic, signal transduction, and developmental gene networks. A large set of transcription factors, which may be potential targets for crop breeding, was identified. In addition, gene expression profiles and changes in specific metabolites were also correlated. The results show that several dicotyledonous plant responses to Pi starvation are conserved in maize, but that some genetic responses appear to be more specific and that Pi deficiency leads to a shift in the recycling of internal Pi in maize roots. Ultimately, this work provides a more comprehensive view of Pi-responses in a model for economically important cereals and also sets a framework to produce Pi-specific maize microarrays to study the changes in global gene expression between Pi-efficient and Pi-inefficient maize genotypes.