Extraction study of algal pigments in river bed sediments by applying factorial designs
Extraction study of algal pigments in river bed sediments by applying factorial designs
复制标题
DOI：
10.1016/j.talanta.2007.02.006
复制
发表时间：
2007-06-15
期刊：
影响因子：
6.1
通讯作者：
Barral, M. T.
中科院分区：
文献类型：
--
作者：
Devesa, R.;Moldes, A.;Barral, M. T.
河床沉积物中的植物色素含量随有效营养盐浓度的变化而变化，是反映沉积物营养状态和环境质量的一个有用指标。本文采用三种不完全析因设计，分别用二甲基亚砜（DMSO）、甲醇和丙酮从河流沉积物中提取藻类色素。本研究中使用的自变量及其变化范围为：浸提剂：沉淀物比（1-5 mL/g）、温度（40-80 ℃）和处理持续时间（30-90 min）。获得的实验数据（叶绿素-a，叶绿素-b和总类胡萝卜素含量）允许开发的经验模型，为每种植物色素，描述操作和实验变量之间的相互关系的方程，包括线性，相互作用和二次项。实验结果表明，与DMSO相比，甲醇和丙酮对河流沉积物中植物色素的提取效果较差。该模型预测，在38 ℃下使用3.6 mL DMSO/g沉淀物在40 min内进行的单次提取中，DMSO释放了测试范围内89%的叶绿素a，而提取叶绿素b和总类胡萝卜素的最佳条件分别是使用3.6-3.7 mL DMSO/g沉淀物在64-67 ℃下持续50-51 min，在一次提取中释放76%的叶绿素-b和100%的总类胡萝卜素。(C)2007 Elsevier B. V.保留所有权利。
Phytopigment content of riverbed sediments changes in response to different concentrations of available nutrients and results in a useful descriptor of their trophic state and environmental quality. In this work three incomplete factorial designs of experiments were carried out for extracting algal pigments from river sediments using dimethylsulphoxide (DMSO), methanol or acetone. The independent variables used in this study and their variation limits were: extractant: sediment ratio (1-5 mL/g), temperature (40-80 degrees C) and duration of treatments (30-90 min). The experimental data obtained (chlorophyll-a, chlorophyll-b and total carotenoids content) allowed the development of empirical models for each phytopigment, describing the interrelationship between operational and experimental variables by equations, including linear, interaction and quadratic terms. The experimental results obtained showed that methanol and acetone were less effective extractants for phytopigments from river sediments when compared to DMSO. The model predicted that in a single extraction using 3.6 mL of DMSO/g of sediment at 38 degrees C during 40 min, DMSO releases the 89% of chlorophyll-a in the range tested, whereas the best conditions for extracting chlorophyll-b and total carotenoids are achieved using 3.6-3.7 mL of DMSO/g of sediment, at 64-67 degrees C for 50-51 min, respectively, releasing the 76% of the chlorophyll-b and the 100% of the total carotenoids in a single extraction. (C) 2007 Elsevier B.V. All rights reserved.