Variability in response to UV-B among species and strains of Metarhizium isolated from sites at latitudes from 61°N to 54°S
Variability in response to UV-B among species and strains of Metarhizium isolated from sites at latitudes from 61°N to 54°S
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DOI：
10.1006/jipa.2001.5048
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发表时间：
2001-08-01
影响因子：
3.4
通讯作者：
Roberts, DW
中科院分区：
文献类型：
--
作者：
Braga, GUL;Flint, SD;Roberts, DW
研究了从北纬 61 度到南纬 54 度分离的 2 个绿僵菌菌株、26 个 M. anisopliae 菌株、1 M. 黄绿菌株和 1 M. Taii 菌株中 920 和 1200 mW m(-2) 辐照度（生物有效加权辐照度）的影响。将分生孢子暴露于 UV-B 1 至 6 小时，随后检查相对百分比可培养性。对于较低辐照度，暴露期结束时接收的总剂量范围为 3.3 至 19.9 kJ m(-2)，对于较高辐照度，接收总剂量范围为 4.3 至 25.9 kJ m(-2)。辐照度值和剂量均符合环境实际，即使在温带地区也可以观察到。对于来自 1 小时和 2 小时暴露的数据的两个辐照度水平，比较了原产地纬度和 UV-B 耐受性之间的关系。暴露在两种辐照度下都大大降低了所有菌株的相对可培养性百分比。不同菌株对 UV-B 的耐受性差异很大，并且在所有暴露时间段后，两种辐照度的耐受性都有很大差异。照射 1 小时后，可检测到两种辐照度水平之间的差异，并且这种差异在较长时间的照射下会放大。暴露 1 小时和 2 小时后，观察到 UV-B 耐受性随纬度增加而降低的显着二次关系。对于两个辐照度水平，关系的形状没有不同。此外，我们还研究了1200 MW m(-2) 辐照度对1 M. album 菌株、7 M. anisopliae 菌株和1 M. Taii 菌株中分生孢子萌发时间的影响。暴露于 UV-B 会延迟所有菌株存活分生孢子的萌发。一般来说，发芽延迟与剂量成正比。 (C) 2001 年学术出版社。
The effects of irradiances of 920 and 1200 mW m(-2) (biologically effective weighted irradiance) were examined in 2 Metarhizium album strains, 26 M. anisopliae strains, 1 M. flavoviride strain, and 1 M. taii strain isolated from sites located at latitudes from 61 degreesN to 54 degreesS. Conidia were exposed to UV-B from 1 to 6 h and subsequently examined for relative percentage culturability. Total dosage received at the end of the exposure periods ranged from 3.3 to 19.9 kJ m(-2) for the lower irradiance and from 4.3 to 25.9 kJ m(-2) for the higher irradiance. Both the irradiance values and the doses are environmentally realistic and can be observed even in temperate regions. The relationships between latitude of origin and UV-B tolerance were compared for the two levels of irradiance for the data from 1 and 2 h exposure. Exposure to both irradiances drastically reduced the relative percentage culturability of all strains. Tolerance to UV-B varied widely among strains and high variation was observed for both irradiances after all periods of exposure. After 1 h of exposure, a difference between the two irradiance levels was detectable, and this difference was magnified at longer irradiations. A significant quadratic relationship of decreasing UV-B tolerance with increasing latitude was observed after exposure of I and 2 h. The shape of the relationship did not differ for the two levels of irradiance. Also, we studied the effect of 1200 MW m(-2) irradiance on conidial germination time in 1 M. album strain, 7 M. anisopliae strains, and 1 M. taii strain. Exposure to UV-B delayed the germination of surviving conidia of all strains. In general, the delay in germination was directly proportional to the dose. (C) 2001 Academic Press.