Neuronal expression of a Caenorhabditis elegans elav-like gene and the effect of its ectopic expression
Neuronal expression of a Caenorhabditis elegans elav-like gene and the effect of its ectopic expression
复制标题
DOI：
10.1006/bbrc.1999.0957
复制
发表时间：
1999-07-14
影响因子：
3.1
通讯作者：
Sakamoto, H
中科院分区：
文献类型：
--
作者：
Fujita, M;Kawano, T;Sakamoto, H
我们检测了秀丽隐杆线虫（C. elegans）elav样基因，我们将其命名为elr-1。elr-1基因编码一个预测的456个氨基酸的蛋白质，含有三个推定的RNA结合结构域，属于ELAV家族，在功能上参与神经元分化。北方印迹分析表明，elr-1 mRNA水平的发育调控。在elr-1启动子控制下的elr-1：：gfp报告基因在神经环附近的环神经节、腹神经索（VNC）以及肛门前和腰神经节中特异性表达。在VNC中，GFP阳性细胞显示为产生乙酰胆碱的运动神经元，其数量随着发育的进行而增加，表明elr-1在成熟神经元中表达。ELR-1蛋白在L4幼虫和成虫阶段的异位表达，而不是更早的阶段，导致不可逆的死亡，伴随着不协调的运动（Unc），明确（Clr），和产卵缺陷（Egl）的表型，这是经常观察到的突变体与神经元缺陷。这些结果表明，ELR-1可能在C.优雅的(C)北京：科学出版社.
We examined the expression of a Caenorhabditis elegans (C. elegans) elav-like gene, which we designated elr-1. The elr-1 gene encodes a predicted 456-amino-acid protein containing three putative RNA-binding domains and belongs to the ELAV family, which is functionally involved in neuronal differentiation. Northern blot analysis suggested that the levels of elr-1 mRNA are regulated developmentally. A elr-1::gfp reporter gene under the control of the elr-1 promoter was expressed specifically in the ring ganglia near the nerve ring, the ventral nerve cord (VNC),and the pre-anal and lumbar ganglia. in the VNC, GFP-positive cells were shown to be acetylcholine-producing motor neurons which increased in number as development proceeded, suggesting that elr-1 is expressed in mature neurons. Ectopic expression of ELR-1 protein at the L4 larval and adult stages, but not earlier stages, caused irreversible death, accompanied by uncoordinated movement (Unc), clear (Clr), and egg-laying defective (Egl) phenotypes, which are often observed in mutants with neuronal defects. These results suggest that ELR-1 may have important functions in specific mature neurons in C. elegans. (C) 1999 Academic Press.