Oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cells.
Oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cells.
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油酸通过改变卵巢颗粒细胞脂滴中储存的脂质类型来减少类固醇生成
DOI：
10.1186/s40104-021-00660-5
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发表时间：
2022-02-08
影响因子：
7
通讯作者：
Chu G
中科院分区：
文献类型：
--
作者：
Zhou X;Mo Z;Li Y;Huang L;Yu S;Ge L;Hu Y;Shi S;Zhang L;Wang L;Gao L;Yang G;Chu G
研究背景油酸是存在于处于负能量平衡状态的家畜体内的一种丰富的游离脂肪酸，它可能对雌性生殖和生育力产生不利影响。油酸诱导牛颗粒细胞中的脂质积聚，这导致泡沫细胞样形态和类固醇生成减少。然而，为什么油酸增加脂质积累，但减少类固醇合成仍不清楚。本研究主要探讨油酸对脂质类型和类固醇生成的影响。结果油酸以浓度依赖性方式增加脂质蓄积，主要增加甘油三酯水平，降低胆固醇酯水平。油酸还导致体外培养的猪颗粒细胞中雌二醇和孕酮的产生下降。此外，油酸上调CD 36和二酰基甘油酰基转移酶2的表达，但下调3-羟基-3-甲基戊二酰辅酶A还原酶、清道夫受体B类成员1和乙酰辅酶A乙酰转移酶2以及类固醇生成相关基因的表达，包括细胞色素P450家族11亚家族A成员1，细胞色素P450家族19亚家族A成员1和3以及类固醇生成急性调节蛋白在mRNA和蛋白质水平。富含油酸的饮食还提高了雌性小鼠卵巢组织中的甘油三酯水平，降低了胆固醇水平，导致雌二醇水平低于对照组。与对照组相比，减少发情天数和窦卵泡和黄体的数量，以及闭锁卵泡的数量增加，被发现在油酸喂养的雌性小鼠。结论油酸改变了储存在卵巢颗粒细胞的脂滴中的脂质类型，并导致类固醇合成减少。这些结果提高了我们对处于负能量平衡状态的牲畜生育力下降的理解。
BackgroundOleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state, and it may have detrimental effects on female reproduction and fertility. Oleic acid induces lipid accumulation in bovine granulosa cells, which leads to a foam cell-like morphology and reduced steroidogenesis. However, why oleic acid increases lipid accumulation but decreases steroidogenesis remains unclear. This study focused on oleic acid’s effects on lipid type and steroidogenesis.ResultsOleic acid increased the lipid accumulation in a concentration-dependent manner and mainly increased the triglyceride level and decreased the cholesterol ester level. Oleic acid also led to a decline in estradiol and progesterone production in porcine granulosa cells in vitro. In addition, oleic acid up-regulated the expression ofCD36and diacylglycerol acyltransferase 2, but down-regulated the expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, scavenger receptor class B member 1 and acetyl-Coenzyme A acetyltransferase 2, as well as steroidogenesis-related genes, including cytochrome P450 family 11 subfamily A member 1, cytochrome P450 family 19 subfamily A member 1 and 3 as well as steroidogenic acute regulatory protein at the mRNA and protein levels. An oleic acid-rich diet also enhanced the triglyceride levels and reduced the cholesterol levels in ovarian tissues of female mice, which resulted in lower estradiol levels than in control-fed mice. Compared with the control, decreases in estrus days and the numbers of antral follicles and corpora lutea, as well as an increase in the numbers of the atretic follicles, were found in the oleic acid-fed female mice.ConclusionsOleic acid changed the lipid type stored in lipid droplets of ovarian granulosa cells, and led to a decrease in steroidogenesis. These results improve our understanding of fertility decline in livestock that are in a negative energy-balance state.
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DOI：
10.3791/4389
发表时间：
2012-09-15
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
McLean AC;Valenzuela N;Fai S;Bennett SA
通讯作者：
Bennett SA
影响因子：
3.8
作者：
Leroy, JLMR;Vanholder, T;Van Soom, A
通讯作者：
Van Soom, A
影响因子：
3.8
作者：
Myers, M;Britt, KL;Kerr, JB
通讯作者：
Kerr, JB
影响因子：
3.6
作者：
Aardema H;van Tol HTA;Wubbolts RW;Brouwers JFHM;Gadella BM;Roelen BAJ
通讯作者：
Roelen BAJ
影响因子：
4.5
作者：
Ma S;Yang D;Li D;Tang B;Yang Y
通讯作者：
Yang Y