Interleukin-induced increase in Ia expression by normal mouse B cells.
Interleukin-induced increase in Ia expression by normal mouse B cells.
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DOI：
10.1084/jem.160.3.679
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发表时间：
1984-09-01
期刊：
影响因子：
--
通讯作者：
Cambier JC
中科院分区：
文献类型：
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作者：
Roehm NW;Leibson HJ;Zlotnik A;Kappler J;Marrack P;Cambier JC
巨噬细胞瘤细胞系P388D_1(P388SN)的构成培养上清(SN)和刀豆蛋白A(ConA)诱导的T细胞杂交瘤FS6-14.13细胞培养上清(FS6 Con A SN)均含有非特异性因子，可诱导正常静息B细胞以剂量依赖的方式增加Ia的表达。在连续6个实验中，P388 SN诱导Ia表达的相对增加为4.9+/-0.9，FS6 ConA SN诱导的Ia表达相对增加为10.7+/-1.5，两种制剂的组合诱导Ia表达的相对增加为13.0+/-1.7。在几乎所有的B细胞中都观察到Ia表达的增加，在培养的24小时内达到最高水平。在没有配体受体Ig交联提供的辅助信号的情况下，尽管几乎所有对照B细胞在没有因子的情况下培养，但IL诱导的B细胞Ia表达增加，仍然保持在G0。这些结果表明，在正常的静息B细胞上表达至少部分白介素类的功能性受体，在没有额外激活信号的情况下，它们的作用可以明显地体现出来。非特异性因子制剂诱导的Ia表达增加与B细胞对T细胞杂交瘤的抗原提呈能力增强有关。然而，FS6 ConA SN的活性与B细胞生长因子活性相关，重组DNA技术制备的白细胞介素2(IL-2)或干扰素-γ没有观察到B细胞Ia表达的增加。
The constitutive culture supernatant (SN) of the macrophage tumor line P388D1 (P388 SN) and the concanavalin A (Con A)-induced culture supernatant of the T cell hybridoma FS6-14.13 (FS6 Con A SN) were shown to contain nonspecific factors capable of inducing increased Ia expression by normal resting B cells in a dose-dependent manner. In six consecutive experiments the relative increase in Ia expression induced by P388 SN was 4.9 +/- 0.9, with FS6 Con A SN 10.7 +/- 1.5, and with a combination of both preparations 13.0 +/- 1.7. This increase in Ia expression was observed to occur in virtually all the B cells, reaching maximum levels within 24 h of culture. The interleukin-induced increase in B cell Ia expression occurred in the absence of ancillary signals provided by ligand-receptor Ig cross-linking and despite the fact that virtually all the control B cells, cultured in the absence of factors, remained in G0. These results suggest that functional receptors for at least some interleukins are expressed on normal resting B cells and their effects can be manifest in the absence of additional activating signals. The increased Ia expression induced by the nonspecific factor preparations was shown to be correlated with enhanced antigen- presenting capacity by the B cells to T cell hybridomas. The nature of the interleukins responsible for these effects remains to be definitively determined, however, the activity of FS6 Con A SN was shown to correlate with B cell growth factor activity and increased B cell Ia expression was not observed using interleukin 2 (IL-2) or interferon-gamma, prepared by recombinant DNA technology.