Direct IBD mapping: identical-by-descent mapping without genotyping.

Direct IBD mapping: identical-by-descent mapping without genotyping.
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直接 IBD 作图:无需基因分型的同源作图。

DOI:
10.1016/j.ygeno.2003.08.002
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发表时间:
2004
期刊:
影响因子:
4.4
通讯作者:
Cheung,VivianG
Cheung,VivianG
中科院分区:
生物学3区
文献类型:
--
作者:
Smirnov,Denis;Bruzel,Alan;Morley,Michael;Cheung,VivianG

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Direct identical-by-descent (IBD) mapping is a technique, that combines genomic mismatch scanning (GMS) and DNA microarray technology, for mapping regions shared IBD between two individuals without locus-by-locus genotyping or sequencing. The lack of reagents has limited its widespread application. In particular, two key reagents have been limiting, 1) mismatch repair proteins MutS, L and H, and 2) genomic microarrays for identifying the genomic locations of the GMS-selected IBD fragments. Here, we describe steps that optimized the procedure and resources that will facilitate the development of direct IBD mapping.
大肠杆菌 K-12 的 His6-MutH、His6-MutL 和 His6-MutS 修复蛋白的一步纯化。
DOI: --
发表时间: 1995
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