The type 1 equilibrative nucleoside transporter regulates anxiety-like behavior in mice
The type 1 equilibrative nucleoside transporter regulates anxiety-like behavior in mice
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DOI：
10.1111/j.1601-183x.2007.00311.x
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发表时间：
2007-11-01
影响因子：
2.5
通讯作者：
Choi, D.-S.
中科院分区：
文献类型：
--
作者：
Chen, J.;Rinaldo, L.;Choi, D.-S.
激活大脑中的腺苷受体可以减少动物和人类的焦虑行为。因为核苷转运体调节腺苷水平，我们使用缺乏1型平衡核苷转运体(ENT1)的小鼠来研究ENT1是否有助于焦虑样行为。与野生型小鼠相比，ENT1基因缺失的小鼠在开阔的田野中心停留的时间更长。在高架的Plus迷宫中，ENT1缺失的小鼠进入开放的手臂的频率更高，探索的时间也更长。与野生型小鼠相比，ENT1基因缺失的小鼠也花了更多的时间探索明暗盒子的亮面。在C57BL/6J小鼠杏仁核内微量注射ENT1特异性拮抗剂--硝基苄基硫代肌苷(NBT硫代肌苷)，可减少在旷野和高架+迷宫中的焦虑样行为。这些发现表明，杏仁核ENT1调节焦虑样行为。ENT1可能是治疗焦虑症的药物靶点。
Activation of adenosine receptors in the brain reduces anxiety-like behavior in animals and humans. Because nucleoside transporters regulate adenosine levels, we used mice lacking the type 1 equilibrative nucleoside transporter (ENT1) to investigate whether ENT1 contributes to anxiety-like behavior. The ENT1 null mice spent more time in the center of an open field compared with wild-type littermates. In the elevated plus maze, ENT1 null mice entered more frequently into and spent more time exploring the open arms. The ENT1 null mice also spent more time exploring the light side of a light-dark box compared with wild-type mice. Microinjection of an ENT1-specific antagonist, nitrobenzylthioinosine (nitrobenzylmercaptopurine riboside), into the amygdala of C57BL/6J mice reduced anxiety-like behavior in the open field and elevated plus maze. These findings show that amygdala ENT1 modulates anxiety-like behavior. The ENT1 may be a drug target for the treatment of anxiety disorders.