Immunohistochemical evidence of rapid extracellular matrix remodeling after iron-particle irradiation of mouse mammary gland
Immunohistochemical evidence of rapid extracellular matrix remodeling after iron-particle irradiation of mouse mammary gland
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DOI：
10.2307/3579170
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发表时间：
1996-02-01
影响因子：
3.4
通讯作者：
BarcellosHoff, MH
中科院分区：
文献类型：
--
作者：
Ehrhart, EJ;Gillette, EL;BarcellosHoff, MH
与稀疏电离辐射相比，高LET辐射具有独特的物理和生物特性。最近的研究表明，稀疏的电离辐射迅速改变了几种组织中的细胞外基质表达的模式，但很少有人知道重离子辐射的影响。本研究探讨密集电离辐射诱导的变化，在乳腺细胞外基质定位的成年雌性BALB/c小鼠全身照射后，与0.8戈伊600 MeV的铁粒子。基底膜和间质细胞外基质蛋白的乳腺基质映射相对于时间postirradiation使用免疫荧光。在1天内在脂肪基质中诱导胶原III，持续增加至第9天，并在第14天消退。免疫反应性腱生蛋白在第1天在上皮中诱导，在第5-9天在上皮-基质界面处是明显的，并且作为基底膜下的浓缩层持续到第14天。这些发现与伽马辐照引起的类似变化平行，但表现出不同的发病和慢性。与此相反，上皮基底膜的完整性，这是不受稀疏的电离辐射，被破坏的铁粒子照射。层粘连蛋白的免疫反应是轻度不规则的，在1小时postirradiation和显示不连续性和增厚，从第1天到第9天。第14天恢复连续性。因此，高LET辐射，如稀疏电离辐射，诱导基质细胞外基质的快速重塑，但也似乎改变上皮基底膜的完整性，这是上皮细胞增殖和分化的重要调节因子。(C)1996年，辐射研究学会
High-LET radiation has unique physical and biological properties compared to sparsely ionizing radiation. Recent studies demonstrate that sparsely ionizing radiation rapidly alters the pattern of extracellular matrix expression in several tissues, but little is known about the effect of heavy-ion radiation. This study investigates densely ionizing radiation-induced changes in extracellular matrix localization in the mammary glands of adult female BALB/c mice after whole-body irradiation with 0.8 Gy 600 MeV iron particles. The basement membrane and interstitial extracellular matrix proteins of the mammary gland stroma were mapped with respect to time postirradiation using immunofluorescence. Collagen III was induced in the adipose stroma within 1 day, continued to increase through day 9 and was resolved by day 14. Immunoreactive tenascin was induced in the epithelium by day 1, was evident at the epithelial-stromal interface by day 5-9 and persisted as a condensed layer beneath the basement membrane through day 14. These findings parallel similar changes induced by gamma irradiation but demonstrate different onset and chronicity. In contrast, the integrity of epithelial basement membrane, which was unaffected by sparsely ionizing radiation, was disrupted by iron-particle irradiation. Laminin immunoreactivity was mildly irregular at 1 h postirradiation and showed discontinuities and thickening from days 1 to 9. Continuity was restored by day 14. Thus high-LET radiation, like sparsely ionizing radiation, induces rapid remodeling of the stromal extracellular matrix but also appears to alter the integrity of the epithelial basement membrane, which is an important regulator of epithelial cell proliferation and differentiation. (C) 1996 by Radiation Research Society