Cross-reactive and group-specific immune responses to a neutralizing epitope of the human respiratory syncytial virus fusion protein
Cross-reactive and group-specific immune responses to a neutralizing epitope of the human respiratory syncytial virus fusion protein
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DOI：
10.1007/s007050050143
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发表时间：
1997-01-01
影响因子：
2.7
通讯作者：
Pothier, P
中科院分区：
文献类型：
--
作者：
Corvaisier, C;Bourgeois, C;Pothier, P
对一种对应于B组呼吸道合胞病毒（RS）融合蛋白第205 - 225位氨基酸的合成肽的免疫反应在小鼠和兔子中进行了研究，并与A组RS病毒的一种类似肽进行了比较。肽205 - 225（B）可被单克隆抗体RS - 348识别，在小鼠和兔子中均具有免疫原性，A组毒株融合蛋白的肽205 - 225也是如此。肽205 - 225（B）诱导了增殖性T细胞反应，表明在B组病毒融合蛋白的该区域存在一个T细胞表位。当用同源或异源肽对小鼠进行初次免疫时，两种肽都能够诱导T细胞交叉反应性增殖。使用合成肽或全病毒（来自A组和B组）作为抗原进行酶联免疫吸附测定（ELISA）。小鼠抗肽血清可识别同源和异源肽。在RS病毒毒株中也观察到了类似的模式。在间接免疫荧光试验中，两种抗肽兔血清都能识别感染了A组或B组RS病毒的人鼻上皮细胞。相比之下，虽然A组的抗肽205 - 225兔血清能够中和A组和B组的RS病毒，但B组的抗肽205 - 225兔血清虽然能够中和B组毒株，却无法中和A组病毒。这些结果与儿童初次感染RS病毒后的免疫反应相似。
Immune responses to a synthetic peptide corresponding to aminoacids 205-225 of the fusion protein from group B respiratory syncytial (RS) virus, were studied in mice and rabbits, and compared to a similar peptide from group A RS virus. Peptide 205-225 (B) was recognized by monoclonal antibody RS-348, and was immunogenic in both mice and rabbits, as was peptide 205-225 from the fusion protein of a group A strain. Peptide 205-225 (B) induced a proliferative T-cell response, demonstrating the existence of a T-cell epitope in this region of the fusion protein of group B viruses. Both peptides were able to induce a T-cell cross-reactive proliferation when mice were primed with either the homologous or the heterologous peptide. ELISA were performed using synthetic peptides or whole virus (from group A and B) as antigens. Mice anti-peptide sera recognized both homologous and heterologous peptides. A similar pattern was observed with RS virus strains. In indirect immunofluorescence assays, both anti-peptide rabbit sera recognized human nasal epithelial cells infected with A or B strains of RS virus. In contrast, while anti-peptide 205-225 rabbit serum from group A neutralized group A and B strains of RS virus, anti-peptide 205-225 rabbit serum from group B was unable to neutralize a group A virus, although it neutralized a group B strain. These results are similar to the immune response observed in children following primary RS virus infection.