Tomographic in vivo microscopy for the study of lung physiology at the alveolar level
Tomographic in vivo microscopy for the study of lung physiology at the alveolar level
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DOI：
10.1038/s41598-017-12886-3
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发表时间：
2017-10-02
影响因子：
4.6
通讯作者：
Stampanoni, Marco
中科院分区：
文献类型：
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作者：
Lovric, Goran;Mokso, Rajmund;Stampanoni, Marco
肺是哺乳动物呼吸系统的重要组成部分，这反映在肺衰竭仍然是世界范围内发病率和死亡率的主要原因之一。建立吸气和呼气的宏观观察与微观尺度上发生的过程之间的联系对于理解基本的生理和病理过程至关重要。在这里，我们首次展示了基于同步加速器的肺层析成像，其像素尺寸降至微米，从而首次深入了解高分辨率肺结构。我们报告了在肺泡尺度上研究肺膨胀模式的方法学能力及其在解决肺生理学中仍未解决的问题方面的潜力。作为第一个应用，我们确定了肺泡水平的异质性扩张模式，并评估了体内和死后立即状态之间肺的首次比较。
Lungs represent the essential part of the mammalian respiratory system, which is reflected in the fact that lung failure still is one of the leading causes of morbidity and mortality worldwide. Establishing the connection between macroscopic observations of inspiration and expiration and the processes taking place at the microscopic scale remains crucial to understand fundamental physiological and pathological processes. Here we demonstrate for the first time in vivo synchrotron-based tomographic imaging of lungs with pixel sizes down to a micrometer, enabling first insights into high-resolution lung structure. We report the methodological ability to study lung inflation patterns at the alveolar scale and its potential in resolving still open questions in lung physiology. As a first application, we identified heterogeneous distension patterns at the alveolar level and assessed first comparisons of lungs between the in vivo and immediate post mortem states.