Genetic variation at the mitochondrial large-subunit rRNA locus of Pneumocystis isolates from simian immunodeficiency virus-infected rhesus macaques.
Genetic variation at the mitochondrial large-subunit rRNA locus of Pneumocystis isolates from simian immunodeficiency virus-infected rhesus macaques.
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从猿猴免疫缺陷病毒感染的恒河猴中分离出的肺孢子菌线粒体大亚基 rRNA 位点的遗传变异。
DOI：
10.1128/cdli.10.6.1037-1042.2003
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Board,KathrynF
中科院分区：
文献类型：
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作者：
Norris,KarenA;Wildschutte,Hans;Franko,Jennifer;Board,KathrynF
对猴免疫缺陷病毒(SIV)和卡氏肺孢子虫共感染猕猴的肺孢子虫线粒体大亚基(Mt LSU)rRNA基因的一段核苷酸序列进行了测定。在检测的12个分离株中，有3个被发现是相同的，其他的显示出很大的序列差异，变异之间的差异高达13%。我们确定了两种在几个位点上不同的一般序列类型，包括保守的26个核苷酸插入。四只猴子同时发现了两种肺孢子虫病变种，这表明这是一种混合感染。恒河猴来源的肺孢子虫LSU rRNA基因序列与其他宿主来源的肺孢子虫LSU rRNA基因同源序列有很大差异。对来自恒河猴和其他几种哺乳动物宿主的肺孢子虫mt LSU rRNA基因的分析支持了恒河猴肺孢子虫病与人类肺孢子虫关系最密切的观察结果。此外，数据还证实了mt LSU rRNA基因是SIV-恒河猴肺孢子虫感染模型的传播和流行病学研究的信息位点。
The nucleotide sequences of a segment of thePneumocystismitochondrial large-subunit (mt LSU) rRNA gene from rhesus macaques coinfected with simian immunodeficiency virus (SIV) andPneumocystis cariniiwere examined. Of 12 isolates examined, 3 were found to be identical and the others showed substantial sequence variation, with up to 13% divergence among variants. We identified two general sequence types that differed at several sites, including a conserved 26-nucleotide insertion. Four monkeys had evidence of twoPneumocystisvariants present simultaneously, indicative of a mixed infection. There was a high degree of variance between the rhesus macaque-derivedPneumocystismt LSU rRNA gene sequence and the cognate sequences inPneumocystisorganisms derived from other hosts. Analysis of the mt LSU rRNA genes ofPneumocystisorganisms derived from rhesus macaques and several other mammalian hosts supports the observation that rhesus macaque-derivedPneumocystisis most closely related to human-derivedPneumocystis. In addition, the data identify the mt LSU rRNA gene as an informative locus for transmission and epidemiological studies of the SIV-rhesus macaque model ofPneumocystisinfection.