Digital Data Storage Using DNA Nanostructures and Solid-State Nanopores
Digital Data Storage Using DNA Nanostructures and Solid-State Nanopores
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DOI：
10.1021/acs.nanolett.8b04715
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发表时间：
2019-02-01
期刊：
影响因子：
10.8
通讯作者：
Keyser, Ulrich F.
中科院分区：
文献类型：
--
作者：
Chen, Kaikai;Kong, Jinglin;Keyser, Ulrich F.
固态纳米孔是阅读分子三维形状的强大工具，可以将分子结构信息转化为电信号。在这里，我们展示了一个高分辨率的集成纳米孔系统，用于识别DNA纳米结构，具有区分连接的短DNA发夹的能力，只有一个干长度的差异为8 bp沿着的DNA双链命名的DNA载体。使用我们的平台，我们可以读取多达112个DNA发夹，其分离距离为114 bp，附着在携带数字信息的DNA载体上。我们的编码策略允许创建大小高达5 × 10（33）（2（112））的分子库，该分子库仅由数百种用于数据存储的基本分子构建，并且具有通过连接多个DNA载体进行扩展的潜力。我们的平台提供了一种基于纳米孔和DNA纳米结构的数据存储方法，具有方便的访问和微型集成的潜力。
Solid-state nanopores are powerful tools for reading the three-dimensional shape of molecules, allowing for the translation of molecular structure information into electric signals. Here, we show a high-resolution integrated nanopore system for identifying DNA nanostructures that has the capability of distinguishing attached short DNA hairpins with only a stem length difference of 8 bp along a DNA double strand named the DNA carrier. Using our platform, we can read up to 112 DNA hairpins with a separating distance of 114 bp attached on a DNA carrier that carries digital information. Our encoding strategy allows for the creation of a library of molecules with a size of up to 5 x 10(33) (2(112)) that is only built from a few hundred types of base molecules for data storage and has the potential to be extended by linking multiple DNA carriers. Our platform provides a nanopore- and DNA nanostructure-based data storage method with convenient access and the potential for miniature-scale integration.