Gap junctional coupling modulates secretion of exocrine pancreas.
Gap junctional coupling modulates secretion of exocrine pancreas.
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间隙连接耦合调节外分泌胰腺的分泌。
DOI：
10.1073/pnas.84.14.4901
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发表时间：
1987
影响因子：
11.1
通讯作者：
Orci,L
中科院分区：
文献类型：
--
作者：
Meda,P;Bruzzone,R;Chanson,M;Bosco,D;Orci,L
对分散的胰腺腺泡细胞进行研究，以评估成体分泌细胞之间的连接偶联功能。在每个腺泡中，未受刺激的对照细胞广泛地耦合到它们的邻近细胞。庚醇的加入使其解偶联，增加了其基础淀粉酶的释放。神经降压素、促胰液素和血管活性肠肽(VIP)刺激淀粉酶的分泌，而不使腺泡细胞解偶联。正庚醇迅速而显著地解偶联神经降压素、分泌素和血管活性肠肽刺激的腺泡细胞，并以相加的方式增加其淀粉酶的分泌。相比之下，氨基甲酰胆碱(卡巴胆碱)的分泌反应不受庚醇的影响。氨基甲酰胆碱是一种促分泌剂，单独作用于非偶联的腺泡细胞。在去除庚醇和恢复正常偶联后，基础以及神经降压素、分泌素和血管活性肠肽刺激的输出量恢复到较低的控制值。这些数据提供了证据，阻断缝隙连接偶联增加了胰腺外分泌的基础分泌，以及腺体对各种促分泌剂的反应。
Dispersed pancreatic acini were studied to assess the function of junctional coupling between adult secretory cells. Nonstimulated control cells were extensively coupled to their neighbors throughout each acinus. Addition of heptanol caused their uncoupling and increased their basal amylase release. Neurotensin, secretin, and vasoactive intestinal peptide (VIP) stimulated amylase secretion without uncoupling acinar cells. Heptanol rapidly and markedly uncoupled the neurotensin-, secretin-, and VIP-stimulated acinar cells and increased their amylase secretion in an additive manner. By contrast, the secretory response to carbamoylcholine (carbachol), a secretagogue that, alone, uncoupled acinar cells, was not affected by heptanol. Basal as well as neurotensin-, secretin-, and VIP-stimulated output returned to the lower control values following removal of heptanol and recovery of normal coupling. The data provide evidence that blockage of gap junctional coupling increases the basal secretion of exocrine pancreas as well as the response of the gland to a variety of secretagogues.