V3 INTERNEURON SUBPOPULATIONS IN THE MOUSE SPINAL CORD UNDERGO DISTINCTIVE POSTNATAL MATURATION PROCESSES
V3 INTERNEURON SUBPOPULATIONS IN THE MOUSE SPINAL CORD UNDERGO DISTINCTIVE POSTNATAL MATURATION PROCESSES
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DOI：
10.1016/j.neuroscience.2015.03.024
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发表时间：
2015-06-04
期刊：
影响因子：
3.3
通讯作者：
Zhang, Y.
中科院分区：
文献类型：
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作者：
Borowska, J.;Jones, C. T.;Zhang, Y.
小鼠在出生后的前 2-3 周内形成负重运动，在此期间，控制运动活动的运动神经元 (MN) 和中间神经元 (IN) 快速成熟。在这项研究中，我们研究了这一时期小鼠脊髓中两个 V3 IN 亚群的成熟情况。为此，我们对来自 Sim1(Cre/+) 的表达 tdTomato 荧光蛋白的脊髓 V3 IN 进行了全细胞膜片钳记录； tdTom 小鼠在出生后第 0 天、第 4 天、第 9 天和第 14 天，并将它们的特性与第 21 天的特性进行了比较。将电生理学与计算分析相结合，我们发现背侧和腹侧 V3 亚群在出生时在生理上是不同的，但 V3 IN 的电生理特性在出生后的前三周内发生显着变化。我们进一步揭示，两个 V3 亚群在成熟过程中都存在多个发育阶段。生理特性的不同发育轨迹也与动物运动行为的变化相一致。这些特性可能反映了 V3 亚群在成熟脊髓运动回路中的差异功能。 (C) 2015 国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Mice develop weight-bearing locomotion within the first 2-3 weeks of birth, a period during which motoneurons (MNs) and interneurons (INs) that control locomotor activities undergo rapid maturation. In this study, we investigate the maturation of two subpopulations of V3 INs in the mouse spinal cord during this period. To do this, we conducted whole-cell patch-clamp recordings of tdTomato fluorescent protein-expressing spinal V3 INs from Sim1(Cre/+); tdTom mice at post-natal day (P) 0, P4, P9 and P14 and compared their properties to those at P21. Combining electrophysiology with computational analyses, we show that dorsal and ventral V3 subpopulations are physiologically distinct at birth, but the electrophysiological properties of V3 INs change significantly during the first three post-natal weeks. We further reveal that there are multiple developmental phases of both V3 subpopulations during the maturation process. The different developmental trajectories of physiological properties also coincide with changes in an animal's locomotor behavior. These properties likely reflect the differential functions of V3 subpopulations in maturing spinal locomotor circuits. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.