MOLECULAR-CLONING OF AN APOLIPOPROTEIN-B MESSENGER-RNA EDITING PROTEIN
MOLECULAR-CLONING OF AN APOLIPOPROTEIN-B MESSENGER-RNA EDITING PROTEIN
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DOI：
10.1126/science.8511591
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发表时间：
1993-06-18
期刊：
影响因子：
56.9
通讯作者：
DAVIDSON, NO
中科院分区：
文献类型：
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作者：
TENG, BB;BURANT, CF;DAVIDSON, NO
哺乳动物载脂蛋白B （apo B）以两种形式存在，每种形式都是单个基因的产物。较短的形式，载脂蛋白B48，由转录后RNA编辑产生，其中胞苷脱氨产生一个UAA终止密码子。从大鼠小肠中分离到一个编码载脂蛋白B信使RNA编辑蛋白（REPR）的全长互补DNA克隆。229个残基蛋白包含一致的磷酸化位点和亮氨酸拉链结构域。HepG2细胞提取物与卵母细胞提取物的REPR混合后获得编辑活性。REPR对载脂蛋白B信使RNA编辑至关重要，REPR的分离和鉴定可能会导致其他真核RNA编辑蛋白的鉴定。
Mammalian apolipoprotein B (apo B) exists in two forms, each the product of a single gene. The shorter form, apo B48, arises by posttranscriptional RNA editing whereby cytidine deamination produces a UAA termination codon. A full-length complementary DNA clone encoding an apo B messenger RNA editing protein (REPR) was isolated from rat small intestine. The 229-residue protein contains consensus phosphorylation sites and leucine zipper domains. HepG2 cell extracts acquire editing activity when mixed with REPR from oocyte extracts. REPR is essential for apo B messenger RNA editing, and the isolation and characterization of REPR may lead to the identification of other eukaryotic RNA editing proteins.