Assessment of cytotoxicity of quantum dots and gold nanoparticles using cell-based. impedance spectroscopy
Assessment of cytotoxicity of quantum dots and gold nanoparticles using cell-based. impedance spectroscopy
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DOI：
10.1021/ac8004555
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发表时间：
2008-07-15
影响因子：
7.4
通讯作者：
Luong, John H. T.
中科院分区：
文献类型：
--
作者：
Male, Keith B.;Lachance, Bernard;Luong, John H. T.
一种基于电细胞-基质阻抗传感（ECIS）的连续在线技术被证明用于测量暴露于纳米材料如量子点（QD）和荧光金纳米颗粒的成纤维细胞V79细胞的浓度和时间响应函数。根据响应函数估计半抑制浓度（ECIS 50），即达到50%细胞毒性反应抑制所需的浓度，以确定测量过程中的细胞毒性。ECIS 50值与使用标准中性红测定法获得的结果一致。用ECIS分析法显示硒化镉量子点具有直接的细胞毒性。对于碲化镉量子点，毒性显著。可以分配到游离镉，虽然额外的毒性可以归因于量子点本身。用磷化铟镓和荧光金纳米颗粒合成的量子点没有细胞毒性。
A continuous online technique based on electric cell-substrate impedance sensing (ECIS) was demonstrated for measuring the concentration and time response function of fibroblastic V79 cells exposed to nanomaterials such as quantum dots (QDs) and fluorescent gold nanoparticles. The half-inhibition concentration, (ECIS50), the required concentration to attain 50% inhibition of the cytotoxic response, was estimated from the response function to ascertain cytotoxicity during the course of measurement. The ECIS50 values agreed well with the results obtained using the standard neutral red assay. Cadmium selenide quantum dots showed direct cytoxicity with the ECIS assay. For the cadmium telluride quantum dots, significant toxicity. could be assigned to free cadmium, although additional toxicity could be attributed to the QDs per se. The QDs synthesized with indium gallium phosphide and the fluorescent gold nanoparticles were not cytotoxic.