In silico estimates of cell electroporation by electrical incapacitation waveforms.
In silico estimates of cell electroporation by electrical incapacitation waveforms.
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通过电失能波形对细胞电穿孔进行计算机估计。
DOI：
10.1109/iembs.2009.5333138
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Weaver,JC
中科院分区：
文献类型：
--
作者：
Gowrishankar,TR;Esser,AT;Smith,KC;Burns,SK;Weaver,JC
我们使用一个系统模型的细胞和电失能（EI）设备波形的近似幅度估计条件，导致响应或不电穿孔（EP）的细胞膜附近的电极。使用电阻负载测量了泰瑟X26和Aegis MK 63器械的单脉冲波形。对于目前的估计，数字化波形的大小根据两个组织穿透电极的径向距离的平方反比进行缩放，近似为半球。然后将相应的组织水平电场用作细胞系统模型的输入。将膜电穿孔（EP）的动态孔模型分配给细胞质膜（PM）上的许多不同位点。EI装置产生足够大的跨膜电压Um（t），使得产生孔，演变成异质和时间依赖性孔群。这些近似响应表明，两种波形均可引起PM EP。EP引起的周围神经损伤是一种可能的副作用。预计泰瑟X26的EP比Aegis MK63更广泛，主要是由于峰值幅度相差约8倍。通过多尺度建模对EI波形进行计算机检查是有必要的，并且可以涉及现在存在并正在迅速改进的全身、组织和细胞水平模型。
We use a system model of a cell and approximate magnitudes of electrical incapacitation (EI) device waveforms to estimate conditions that lead to responses with or without electroporation (EP) of cell membranes near electrodes. Single pulse waveforms of Taser X26 and Aegis MK63 devices were measured using a resistive load. For the present estimates the digitized waveforms were scaled in magnitude according to the inverse square radial distance from two tissue-penetrating electrodes, approximated as hemispheres. The corresponding tissue level electric fields were then used as inputs to the cell system model. A dynamic pore model for membrane electroporation (EP) was assigned to many different sites on the cell plasma membrane (PM). EI devices generate sufficiently large transmembrane voltage, Um(t), such that pores were created, evolving into a heterogeneous and time-dependent pore population. These approximate responses suggest that both waveforms can cause PM EP. Peripheral nerve damage by EP is a candidate side effect. More extensive EP is expected from the Taser X26 than the Aegis MK63, mainly due to the approximately eight-fold difference in the peak magnitudes. In silico examination of EI waveforms by multiscale modeling is warranted, and can involve whole body, tissue and cell level models that now exist and are rapidly being improved.