Subset-Specific Expression of Toll-Like Receptors by Bovine Afferent Lymph Dendritic Cells.
Subset-Specific Expression of Toll-Like Receptors by Bovine Afferent Lymph Dendritic Cells.
复制标题
DOI：
10.3389/fvets.2017.00044
复制
发表时间：
2017
影响因子：
3.2
通讯作者：
Coffey TJ
中科院分区：
文献类型：
--
作者：
Werling D;Hope JC;Siddiqui N;Widdison S;Russell C;Sopp P;Coffey TJ
在反刍动物系统内，存在几种可能性来产生树突状细胞，其从组织迁移到区域引流淋巴结中作为传入淋巴树突状细胞（ALDC）。在这里，我们分析了Toll样受体（TLR）1-10 mRNA的表达，通过使用定量实时PCR在高度纯化的牛ALDC的子集。由于TLR表达可能受到病原体或疫苗及其佐剂的影响，因此有必要了解稳态系统中表达的TLR，以阐明特定差异并潜在地优化靶向疫苗。在这项研究中，我们评估了四个主要的牛ALDC亚群[cDC 1和cDC 2（亚群2-4）]的TLR表达谱。我们证明了四个亚群之间TLR表达的差异，这可能反映了这些细胞对不同病原体或佐剂的反应能力。
Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymph nodes as afferent lymph dendritic cells (ALDCs). Here, we analyzed toll-like receptor (TLR) 1–10 mRNA expression by using quantitative real-time PCR in highly purified subsets of bovine ALDC. As TLR expression may be influenced by pathogens or vaccines and their adjuvant, it is necessary to understand what TLRs are expressed in a steady-state system to elucidate specific differences and to potentially optimize targeted vaccines. In this study, we have assessed the TLR expression profiles of the four main bovine ALDC subsets [cDC1 and cDC2 (subsets 2–4)]. We demonstrate differences in TLR expression between the four subsets that may reflect the ability of these cells to respond to different pathogens or to respond to adjuvants.