Serotype distribution and antimicrobial susceptibility of USA Streptococcus pneumoniae isolates collected prior to and post introduction of 13-valent pneumococcal conjugate vaccine
Serotype distribution and antimicrobial susceptibility of USA Streptococcus pneumoniae isolates collected prior to and post introduction of 13-valent pneumococcal conjugate vaccine
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DOI：
10.1016/j.diagmicrobio.2014.05.020
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发表时间：
2014-09-01
影响因子：
2.9
通讯作者：
Jones, Ronald N.
中科院分区：
文献类型：
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作者：
Mendes, Rodrigo E.;Costello, Andrew J.;Jones, Ronald N.
本研究评价了2011-2012年期间从美国住院患者（所有年龄组）的血液或下呼吸道标本中培养的肺炎球菌（N = 1190），并将结果与2008年进行的类似研究（N = 694）的结果进行了比较。通过肉汤微量稀释法检测分离株的敏感性，通过cpsB测序确定血清型，并辅以多重PCR和荚膜肿胀试验。2008年和2011-2012年，7价肺炎球菌结合疫苗（PCV 7）类型的相对百分比分别为6.3%和4.9%，最常见的PCV 7血清型（19 F和6 B）分别仅占两个时期所有分离株的3.7%和4.0%。13价肺炎球菌结合疫苗（PCV 13）血清型在2008年占分离株的42.9%，在第二阶段占30.1%，这一下降是由19 A和7 F驱动的。非PCV 13血清群/血清型23 A、15 B/15 C、7 C、8和31增加。当应用肠外（即>= 4 μ g/mL）和口服折点（即>= 0.12 μ g/mL）时，青霉素不敏感率分别为9.6-10.0%和38.9-42.7%。根据MIC 50和MIC 90值，头孢洛林是最有效的药物（
This study evaluated pneumococci cultured from blood or lower respiratory tract specimens from hospitalized patients in the USA (all age groups) during 2011-2012 (N = 1190) and compared findings with those from a similar study performed in 2008 (N = 694). Isolates were tested for susceptibility by broth microdilution and serotypes determined by cpsB sequencing, supplemented with multiplex PCR and capsular swelling assays. Relative percentages of 7-valent pneumococcal conjugate vaccine (PCV7) types were 6.3 and 4.9% in 2008 and 2011-2012, respectively, and the most common PCV7 serotypes (19F and 6B) comprised only 3.7% and 4.0% of all isolates from both periods, respectively. Thirteen-valent pneumococcal conjugate vaccine (PCV13) serotypes represented 42.9% of isolates in 2008 and 30.1% in the second period, and this decrease was driven by 19A and 7F. Non-PCV13 serogroups/serotypes 23A, 15B/15C, 7C, 8, and 31 increased. Penicillin non-susceptibility rates were 9.6-10.0% and 38.9-42.7% when applying the parenteral (i.e. >= 4 mu g/mL) and oral breakpoints (i.e. >= 0.12 mu g/mL), respectively. Ceftaroline was the most potent agent tested based on MIC50, and MIC90 values (