Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus.
Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus.
复制标题
将 CD134 的结构域绘制为猫免疫缺陷病毒的功能受体。
DOI：
10.1128/jvi.00722-06
复制
发表时间：
2006
影响因子：
5.4
通讯作者：
Hosie,MargaretJ
中科院分区：
文献类型：
--
作者：
Willett,BrianJ;McMonagle,ElizabethL;Bonci,Francesca;Pistello,Mauro;Hosie,MargaretJ
CD134的猫科同源物是猫科免疫缺陷病毒(FIV)的主要结合受体，优先靶向激活的CD4+辅助T细胞。然而，FIV的菌株在利用CD134方面有所不同；原型菌株PPR需要猫科动物CD134的第一个富含半胱氨酸的区域(CRD1)中的最小决定簇才能提供近乎最佳的受体功能，而GL8等菌株需要CD134 CRD2中的额外决定因素。我们将这个决定因素映射到CRD2中控制受体与其配体之间相互作用的环；氨基酸取代S78N-S79Y-K80E在猫CD134的背景下恢复了人CD134的CDR2的完整病毒受体活性，酪氨酸-79似乎是恢复受体功能的关键残基。
The feline homologue of CD134 is the primary binding receptor for feline immunodeficiency virus (FIV), targeting the virus preferentially to activated CD4+helper T cells. However, strains of FIV differ in utilization of CD134; the prototypic strain PPR requires a minimal determinant in the first cysteine-rich domain (CRD1) of feline CD134 to confer near-optimal receptor function, while strains such as GL8 require additional determinants in the CD134 CRD2. We map this determinant to a loop in CRD2 governing the interaction between the receptor and its ligand; the amino acid substitutions S78N-S79Y-K80E restored full viral receptor activity to the CDR2 of human CD134 in the context of feline CD134, with tyrosine-79 appearing to be the critical residue for restoration of receptor function.