MULTIFUNCTIONAL HYDROXYCAMPTOTHECIN-CAPPED Fe3O4 NANOPARTICLES FOR INHIBITING OF CANCER DRUG RESISTANCE

MULTIFUNCTIONAL HYDROXYCAMPTOTHECIN-CAPPED Fe3O4 NANOPARTICLES FOR INHIBITING OF CANCER DRUG RESISTANCE
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DOI：
10.1142/s1793292011002937
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发表时间：
2011-12
期刊：
NANO
影响因子：
1.2
通讯作者：
Gen Zhang;Hui Jiang;Xuemei Wang
Gen Zhang;Hui Jiang;Xuemei Wang
中科院分区：
材料科学4区
文献类型：
--
作者：
Gen Zhang;Hui Jiang;Xuemei Wang

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在本研究中，我们探索了羟基喜树碱(HCPT)包裹的多功能Fe3O4纳米粒子对阿霉素耐药K562细胞(KA细胞)的有效靶向、诱导凋亡、在KA细胞膜上打孔、光热治疗和低溶血活性。用透射电子显微镜和高效液相色谱仪对羟基磷灰石功能化的Fe3O4纳米粒子进行了表征。同时，细胞凋亡染色和DNA片段化分析表明，羟基喜树碱修饰的Fe3O4纳米颗粒(HCPT-Fe3O4)处理KA细胞后，细胞凋亡率明显高于单纯羟基喜树碱处理组。此外，我们的观察还证明了利用半导体激光对HCPT-Fe3O4纳米粒子进行光热治疗来杀伤KA细胞的可能性。此外，通过溶血实验考察了羟基喜树碱-Fe3O4纳米粒子对大鼠红细胞的血液相容性。结果表明，在较低浓度下，羟基磷灰石-Fe3O4纳米粒子不会引起团聚行为。由此可见，羟基喜树碱功能化的Fe3O4纳米粒子容易诱导细胞凋亡和较低的溶血活性，可进一步用于肿瘤的有效多模式治疗。
In this study, we have explored the multifunctional hydroxycamptothecin (HCPT)-capped Fe3O4 nanoparticles efficiently targeting adriamycin-resistant K562 cells (KA cells), inducing apoptosis, making hole on KA cell membrane, photothermal therapy and low hemolysis activity. The HCPT functionalized Fe3O4 nanoparticles were characterized by the transmission electron microscopy (TEM) and high performance liquid chromatographic (HPLC) study. Meanwhile, the apoptotic staining and the DNA fragmentation clearly illustrate that the apoptosis rate apparently increased after the treatment with HCPT-capped Fe3O4 (HCPT-Fe3O4) nanoparticles, compared with that of the only HCPT treatment in KA cells. Moreover, our observations also demonstrate the possibility of the photothermal therapy to kill KA cells by using HCPT-Fe3O4 nanoparticles with semiconductor laser. In addition, the blood compatibility of the HCPT-Fe3O4 nanoparticles was tested by hemolysis assay on washing the isolated rat erythrocytes. The results indicate that HCPT-Fe3O4 nanoparticles did not cause aggregation behavior at lower concentration. Thus, it is evident that the HCPT functionalized Fe3O4 nanoparticles could readily induce cellular apoptosis and low hemolysis activity, which could be further utilized for the efficient multimode therapy of cancers.