Capsiate inhibits ultraviolet B-induced skin inflammation by inhibiting Src family kinases and epidermal growth factor receptor signaling
Capsiate inhibits ultraviolet B-induced skin inflammation by inhibiting Src family kinases and epidermal growth factor receptor signaling
复制标题
DOI：
10.1016/j.freeradbiomed.2010.01.034
复制
发表时间：
2010-05-01
影响因子：
7.4
通讯作者：
Kim, Tae-Yoon
中科院分区：
文献类型：
--
作者：
Lee, Eun-Jung;Jeon, Myung-Shin;Kim, Tae-Yoon
辣椒素酯是辣椒素的主要成分之一，无刺激性，存在于甜椒中。本研究旨在探讨辣椒素酯对紫外线B（UVB）诱导的皮肤炎症反应的影响及其分子机制。衣壳预处理的人角质形成细胞抑制细胞内活性氧（ROS），其激活促分裂原活化蛋白激酶和核因子-κ B（NF-κ B）途径。因此，我们确定了capsiate对这些途径的影响。Capsiate抑制UVB诱导的环氧合酶-2（考克斯-2）表达、细胞外信号相关激酶1/2磷酸化、NF-κ B核转位以及促炎细胞因子和强效血管生成因子（包括血管内皮细胞生长因子和基质金属蛋白酶-2（MMP-2）和MMP-9）的表达。此外，capsiate抑制UVB诱导的表皮生长因子受体（EGFR）激活，从而降低促炎细胞因子和血管生成因子的水平。我们还研究了capsiate在体内的光保护作用。辣椒素酯局部治疗显着降低UVB诱导的皮肤损伤，抑制考克斯-2，促炎细胞因子和血管生成因子，包括血小板/内皮细胞粘附分子-1和细胞间粘附分子-1的表达。抑制Src激酶活性和ROS可抑制埃格活化。因此，capsiate可以保护皮肤免受UVB诱导的不良反应，这些结果为了解其对炎症和血管生成的影响提供了分子基础。(C)2010年爱思唯尔公司All rights reserved.
Capsiate, one of the major capsaicinoids, is nonpungent and present in sweet pepper. We investigated the effects of capsiate on the ultraviolet B (UVB)-induced inflammatory response in skin and its molecular mechanisms. Capsiate-pretreated human keratinocytes inhibited intracellular reactive oxygen species (ROS), which activate the mitogen-activated protein kinase and nuclear factor-kappa B (NF-kappa B) pathways. Therefore, we determined the effects of capsiate on these pathways. Capsiate inhibited UVB-induced cyclooxygenase-2 (COX-2) expression, extracellular signal-related kinase 1/2 phosphorylation, nuclear translocation of NF-kappa B, and the expression of proinflammatory cytokines and potent angiogenic factors, including vascular endothelial cell growth factor and matrix metalloproteinase-2 (MMP-2) and MMP-9. In addition, capsiate inhibited UVB-induced epidermal growth factor receptor (EGFR) activation, which reduces the levels of proinflammatory cytokines and angiogenic factors. We also investigated the photoprotective effects of capsiate in vivo. Topical treatment with capsiate significantly decreased UVB-induced skin damage and inhibited the expression of COX-2, proinflammatory cytokines, and angiogenic factors, including platelet/endothelial cell adhesion molecule-1 and intercellular adhesion molecule-1. Inhibition of Src kinase activity and ROS may inhibit the EGER activation. Therefore, capsiate may protect the skin from UVB-induced adverse effects and these results provide a molecular basis for understanding its effects on inflammation and angiogenesis. (C) 2010 Elsevier Inc. All rights reserved.