High genetic diversity of HIV-1 was found in men who have sex with men in Shijiazhuang, China
High genetic diversity of HIV-1 was found in men who have sex with men in Shijiazhuang, China
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DOI：
10.1016/j.meegid.2011.05.017
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发表时间：
2011-08-01
影响因子：
3.2
通讯作者：
Li, Jingyun
中科院分区：
文献类型：
--
作者：
Li, Lin;Lu, Xinli;Li, Jingyun
男男性行为者(MSM)已成为中国艾滋病病毒感染严重的人群之一。然而，关于HIV-1在这一人群中传播的遗传学研究很少。本研究以石家庄市MSM流行毒株中国为研究对象，根据HIV-1的Gag、Poll和部分env基因序列，对其基因特征进行了分析。21名未服药的HIV-1血清阳性患者进入研究。用巢式RT-PCR方法扩增出全长的gag、pol1、部分env基因和部分接近全长的基因组，然后进行测序。CRF01_AE亚型占52.9%，B亚型占35.3%，CRF07_BC亚型占11.8%。系统进化分析表明，本株与北京MSM株亲缘关系较近，而与当地有偿献血员的亲缘关系不大。耐药性监测未发现传播性耐药(TDR)。然而，在B亚型菌株中发现了一些耐药相关的多态位点(V181I和V106I)。这一发现将有助于了解HIV-1在MSM中的分布和演变，并有助于行为改变的干预。(C)2011爱思唯尔B.V.保留所有权利。
Men who have sex with men (MSM) have become one of the populations with severely HIV prevalence in China. However, very few genetic studies have been done on HIV-1 spreading in this population. In this study, the genetic characterization of HIV-1 strains prevalent in the MSM in Shijiazhuang, China, was analyzed basing on the HIV-1 full-length gag, pol, and partial env gene. 21 drug-naive HIV-1 sero-positive patients were enrolled into the study. Full length gag, pol, partial env genes and some near full length genomes were amplified with nest RT-PCR followed by sequencing. Multiple subtypes, including CRF01_AE (52.9%), subtype B (35.3%) and CRF07_BC (11.8%), were found in the population. Phylogenetic analysis showed close relationship between our strains with those from Beijing MSM but not those from paid blood donors of local area. Drug resistance surveillance evaluation showed no transmitted drug resistance (TDR). However, some drug-resistance related polymorphism sites (V181I and V106I) were popularly found in B subtype strains. The findings will contribute to understanding the distribution and evolution of HIV-1 in MSM and also help the behavior change intervention. (C) 2011 Elsevier B.V. All rights reserved.