Detection rates of aphid DNA in the guts of larval hoverflies and potential links to the provision of floral resources.
Detection rates of aphid DNA in the guts of larval hoverflies and potential links to the provision of floral resources.
复制标题
食蚜蝇幼虫肠道中蚜虫 DNA 的检出率以及与花卉资源供应的潜在联系。
DOI：
10.1017/s0007485321001115
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发表时间：
2022
影响因子：
1.9
通讯作者：
Hodgkiss D
中科院分区：
文献类型：
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作者：
Hodgkiss D
食蚜食蚜蚜蝇（双翅目，食蚜蝇科，食蚜蝇亚科）是一系列开花植物，包括水果作物中常见的访花者和蚜虫捕食者。在这里，我们调查是否可以检测到蚜虫猎物的DNA从商业草莓领域的概念证明，分子方法可以用来衡量农业生物防治的食蚜蝇幼虫的肠道内容物。我们使用高通量测序（HTS）的目标昆虫DNA，并将所得数据与包含蚜虫和食蚜蝇DNA序列的参考数据库进行比较。我们探讨了将野花聚乙烯包裹的隧道内可能有蚜虫的DNA检测率在食蚜蝇幼虫的影响。在随机区组实验中，将芫荽（Coriandrum sativum）、田间勿忘我（Myosotis arvensis）和薄荷（Mentha arvensis）植物插入草莓行中。评估了它们对蚜虫DNA检测率的影响。蚜虫的DNA被发现在55 149个标本（37%）验证的方法，原则上测量由食蚜蝇提供的农业服务。有趣的是，检测率较高的地块附近的勿忘我不是比地块香菜，虽然在控制地块的检测率没有显着差异，无论是野花物种。这些研究结果证实，hoverflies捕食蚜虫在英国草莓领域，高温超导是一种可行的方法，确定蚜虫的DNA在捕食性hoverflies。我们评论需要进一步的方法开发，以缩小识别捕食者和猎物。此外，我们提供了一些证据，有一个效果，间作草莓作物与野花，可能会影响蚜虫消费的食蚜蝇幼虫。
Aphidophagous hoverflies (Diptera, Syrphidae, Syrphinae) are common flower visitors and aphid predators in a range of flowering plants, including fruit crops. Here, we investigate whether aphid prey DNA can be detected in the gut contents of hoverfly larvae from a commercial strawberry field as a proof of concept that a molecular approach can be used to measure agricultural biocontrol. We used high-throughput sequencing (HTS) to target insect DNA and compared the resulting data to reference databases containing aphid and hoverfly DNA sequences. We explored what impact incorporating wildflowers within polythene-clad tunnels may have on aphid DNA detection rates in hoverfly larvae. In a randomized block experiment, coriander (Coriandrum sativum), field forget-me-not (Myosotis arvensis) and corn mint (Mentha arvensis) plants were inserted in rows of strawberries. Their effect on aphid DNA detection rates was assessed. Aphid DNA was found in 55 of 149 specimens (37%) validating the method in principle for measuring agricultural services provided by hoverflies. Interestingly, detection rates were higher near plots with forget-me-not than plots with coriander, though detection rates in control plots did not differ significantly from either wildflower species. These findings confirm that hoverflies predate aphids in UK strawberry fields, and that HTS is a viable method of identifying aphid DNA in predatory hoverflies. We comment on the need for further method development to narrow down identifications of both predator and prey. We furthermore provide some evidence that there is an effect of intercropping strawberry crops with wildflowers which may affect aphid consumption in hoverfly larvae.