Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes.
Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes.
复制标题
具有软骨表型的克隆人软骨肉瘤细胞系的建立。
作者：
Masaharu Takigawa;Koji Tajima;H. Pan;M. Enomoto;A. Kinoshita;Fujio Suzuki;Yoshiro Takano;Yoichi Mori
从人软骨肉瘤中建立了一个具有软骨表型和致瘤性的克隆细胞系，培养时间超过3年。在稀疏培养物中，命名为HCS-2/8的克隆系由略微细长的多边形细胞组成，其增殖时间为3.5天。细胞融合时呈多边形至球形。细胞融合后继续缓慢增殖，形成结节，甲苯胺蓝染色呈异染性。结节的结构是三维的;细胞在表面区域是多层的，覆盖着一层厚的细胞外基质，显示异染性。电子显微镜下，类似体内软骨细胞的细胞被细胞外基质包围，细胞外基质由薄的胶原样原纤维和许多细颗粒组成，可能是蛋白多糖。通过[35 S]硫酸盐掺入测定，细胞积极合成蛋白聚糖。由细胞合成的主要蛋白聚糖单体的流体动力学大小是所谓的软骨特异性蛋白聚糖，如通过甘油梯度离心所确定的。免疫染色鉴定II型胶原蛋白，但不是I型胶原蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的胶原的荧光照相和免疫印迹也证明了II型胶原的合成，但不是I型胶原。将HCS-2/8细胞接种到无胸腺小鼠中导致类似于原始肿瘤的软骨肉瘤的形成。HCS-2/8细胞具有这些特性，不仅可用于人软骨细胞分化表型的研究，而且可用于人软骨肉瘤的诊断、治疗和病因学的基础研究。
A clonal cell line with cartilage phenotypes and tumorigenicity during more than 3 years in culture was established from a human chondrosarcoma. In sparse cultures, the clonal line, named HCS-2/8, consisted of slightly elongated polygonal cells, which proliferated with a doubling time of 3.5 days. The cells became polygonal to spherical as they became confluent. After reaching confluence, the cells continued to proliferate slowly and formed nodules, which showed metachromasia when stained with toluidine blue. The nodules were three-dimensional in structure; cells were multilayered in the surface regions, overlying a thick layer of extracellular matrix, which showed metachromasia. Electron microscopically, the cells resembling chondrocytes in vivo were surrounded by an extracellular matrix consisting of thin collagen-like fibrils with numerous fine granules, presumably of proteoglycans. The cells actively synthesized proteoglycans as determined by [35S]sulfate incorporation. The hydrodynamic size of major proteoglycan monomers synthesized by the cells was that of so-called cartilage-specific proteoglycans, as determined by glycerol gradient centrifugation. Immunostaining identified type II collagen but not type I collagen. Fluorography and immunoblotting of collagens separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis also demonstrated the synthesis of type II collagen but not type I collagen. Inoculation of HCS-2/8 cells into athymic mice resulted in the formation of chondrosarcomas that resembled the original tumor. Because of having these characters, HCS-2/8 cells should be useful not only in studies on the differentiated phenotypes of human chondrocytes but also in basic studies on the diagnosis, treatment, and etiology of human chondrosarcomas.
在视黄酸或 5-溴-2-脱氧尿苷中鸡软骨细胞生长过程中协调 IX 型和 II 型胶原合成的调节。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Yasui,N;Benya,PD;Nimni,ME
通讯作者：
Nimni,ME
佝偻病雏鸡骨骺生长软骨中 X 型胶原蛋白的变化。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Reginato,AM;Shapiro,IM;Lash,JW;Jimenez,SA
通讯作者：
Jimenez,SA