Molecular characterization and classification of Stylosanthes mexicana, S. macrocarpa, S. seabrana and S. fruticosa by DNA sequence analysis of two chloroplast regions.
Molecular characterization and classification of Stylosanthes mexicana, S. macrocarpa, S. seabrana and S. fruticosa by DNA sequence analysis of two chloroplast regions.
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DOI：
10.3109/10425179909033948
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
J. Vander Stappen;G. Volckaert
中科院分区：
文献类型：
--
作者：
J. Vander Stappen;G. Volckaert
通过聚合酶链式反应扩增二倍体物种 Stylosanthes mexicana、S.macrocarpa 和 S.seabrana 以及四倍体物种 S.fruticosa 的 trnL (UAA) 内含子和 trnL (UAA) - trnF (GAA) 基因间间隔区，并进行直接 DNA 测序。将这些叶绿体区域与先前确定的 19 个柱花草物种的 DNA 序列进行比较，揭示了与包含二倍体物种 S. hamata、S. calcicola 和四倍体物种 S. scabra 的进化枝的密切关系。结果用于推断该分支的二倍体物种与异源物种 S. scabra 和 S. fruticosa 之间的关系。
The trnL (UAA) intron and trnL (UAA) - trnF (GAA) intergenic spacer region from the diploid species Stylosanthes mexicana, S. macrocarpa and S. seabrana and the tetraploid species S. fruticosa were amplified by polymerase chain reaction and subjected to direct DNA sequencing. Comparison of these chloroplast regions with previously determined DNA sequences of 19 Stylosanthes species revealed a close relationship with a clade containing the diploid species S. hamata, S. calcicola, and the tetraploid species S. scabra. The results were used to infer relationships between the diploid species of this clade, in relation to the alloploid species S. scabra and S. fruticosa.