Specific lysis of human tumor cells by T cells coated with anti-T3 cross-linked to anti-tumor antibody.
Specific lysis of human tumor cells by T cells coated with anti-T3 cross-linked to anti-tumor antibody.
复制标题
涂有与抗肿瘤抗体交联的抗 T3 的 T 细胞对人类肿瘤细胞进行特异性裂解。
DOI：
10.4049/jimmunol.137.7.2069
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发表时间：
1986
影响因子：
4.4
通讯作者：
D. Segal
中科院分区：
文献类型：
--
作者：
P. Perez;J. A. Titus;M. Lotze;F. Cuttitta;D. Longo;E. Groves;H. Rabin;P. Durda;D. Segal
含有与抗靶细胞抗体交联的抗 T3 的异质聚集体已被证明可导致人 T 细胞裂解表达抗靶细胞抗体识别的抗原的靶细胞。在这项研究中，我们测试了靶向人类 T 细胞裂解人类肿瘤细胞的能力，这是将该现象应用于肿瘤免疫治疗的第一步。分析了几种单克隆抗人肿瘤抗体与多种人肿瘤系的结合以及与抗 T3 交联时促进特异性肿瘤细胞裂解的能力。我们发现，与抗肿瘤单克隆抗体交联的抗 T3 导致克隆的人 T 细胞和新鲜外周血 T 细胞以与结合研究预测相同的特异性裂解肿瘤细胞。然后在各种异质聚集体存在的情况下测试外周血T细胞裂解由新鲜肿瘤或新鲜正常组织制备的单细胞悬浮液的能力。这些研究表明，含有与抗肿瘤抗体交联的抗 T3 的杂聚体会导致新鲜人类 T 细胞特异性裂解新鲜肿瘤细胞，但不会（除了一个例外）新鲜正常细胞。
Heteroaggregates containing anti-T3 cross-linked to anti-target cell antibodies have been shown to cause human T cells to lyse target cells that express antigens recognized by the anti-target cell antibody. In this study, we test targeted human T cells for the ability to lyse human tumor cells as a first step toward the application of this phenomenon to tumor immunotherapy. Several monoclonal anti-human tumor antibodies were assayed for binding to a number of human tumor lines and for the ability to promote specific tumor cell lysis when cross-linked with anti-T3. We found that anti-T3 cross-linked to anti-tumor monoclonal antibodies caused cloned human T cells and fresh peripheral blood T cells to lyse the tumor cells with the same specificity as predicted by the binding studies. Peripheral blood T cells were then tested in the presence of various heteroaggregates for the ability to lyse single cell suspensions prepared from fresh tumor or fresh normal tissue. These studies showed that heteroaggregates containing anti-T3 cross-linked to anti-tumor antibody cause fresh human T cells to specifically lyse fresh tumor cells, but not (with one exception) fresh normal cells.