Phylogeography and population structure of the golden monkeys (Rhinopithecus roxellana):: Inferred from mitochondrial DNA sequences
Phylogeography and population structure of the golden monkeys (Rhinopithecus roxellana):: Inferred from mitochondrial DNA sequences
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DOI：
10.1002/ajp.20425
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发表时间：
2007-11-01
影响因子：
2.4
通讯作者：
Wei, Fuwen
中科院分区：
文献类型：
--
作者：
Li, Ming;Liu, Zhijin;Wei, Fuwen
金丝猴(Rhinopithecus Roxellana)是最濒危的灵长类物种之一，因为在过去的400年里，它的分布急剧减少。其种群仅限于中国境内的青林(QL)、四川/甘肃(SG)和神农架(SNJ)三个孤立地区。与中国和越南的其他金鼻猴一样，这个物种的生物学和进化仍然鲜为人知。为探讨不同地理位置的金丝猴种群间的遗传分化和亲缘关系，对60只金丝猴的线粒体DNA控制区(CR)高变区I(HVI)的379bp进行了研究。从3个地区的7个群体中鉴定出12个单倍型。单倍型多样性较高(0.845)，而核苷酸多样性较低(0.0331)。线粒体DNA单倍型中最近的共同祖先(TMRCA)估计生活在大约480万-32万年前。这三个地区中的任何一个地区都没有共享单倍型。系统发育分析和AMOVA分析揭示了这三个地区之间清晰而显著的系统地理结构。然而，只有SG含有两个主要分支的单倍型，这要么表明单倍型的不完全随机排序，要么表明种群分裂和合并的复杂历史阶段。系统地理结构表明，刺梨应被视为三个地区的单独管理单位(MU)。很可能是最近的系统地理历史塑造了金丝猴的遗传分化模式，金丝猴的种群经历了显著的人口波动。
The golden monkey (Rhinopithecus roxellana) is one of the most endangered primate species due to its dramatically shrinking distribution during the past 400 years. Its populations are restricted to three isolated regions, Qinglin (QL), Sichuan/Gansu (SG), and Shennongjia (SNJ) in China. As with other snub-nosed monkeys in China and Vietnam, the biology and evolution of this species is still poorly known. To assess genetic differentiation and explore the relationships among populations of golden monkeys from different geographic locations, 379bp of mitochondrial DNA control region (CR) hypervariable segment I (HVI) was studied from 60 individuals. Twelve haplotypes were identified from seven populations within the three regions. Haplotype diversity was high (0.845), whereas nucleotide diversity among all haplotypes was low (0.0331). The most recent common ancestor (TMRCA) among mtDNA haplotypes was estimated to have lived approximately 0.48-0.32 million years ago. None of the haplotypes is shared among any of the three regions. Phylogenetic analysis and AMOVA revealed clear and significant phylogeographic structure between the three regions. However, only SG contained haplotypes of the two main clades, indicating either incomplete random sorting of haplotypes or a complex history with phases of population subdivisions and merging of populations. The phylogeographic structure implies that R. roxellana should be regarded as separate management units (MUs) for each of the three regions. It is likely that recent phylogeographic history has shaped the pattern of geneticdifferentiation observed in the golden monkey and that its populations have suffered significant demographic fluctuation.