Release of β-glucan from cell walls of starchy endosperm of barley
Release of β-glucan from cell walls of starchy endosperm of barley
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DOI：
10.1094/cchem.2001.78.2.121
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发表时间：
2001-03-01
期刊：
影响因子：
2.4
通讯作者：
Bamforth, CW
中科院分区：
文献类型：
--
作者：
Kanauchi, M;Bamforth, CW
用温水溶解大麦中的β-葡聚糖，增溶作用随温度升高而增加。如果大麦用硫酸脱壳，提取的葡聚糖会大大减少，特别是如果脱壳大麦变性的话。这表明大麦中存在能够溶解葡聚糖的酶。各种纯化的酶促进变性和脱皮大麦中葡聚糖的增溶。这些酶除了内切-β-(1-->；3)(1-->；4)-葡聚糖酶外，还包括内切-木聚糖酶、阿拉伯呋喃糖苷酶、木乙酰酯酶和阿魏酸酯酶。在大麦淀粉胚乳的细胞壁中，阿魏酸和可能的乙酰基是与阿拉伯木聚糖相连的，而不是β-葡聚糖，所以酯酶、木聚糖酶和阿拉伯呋喃糖苷酶溶解葡聚糖的能力表明细胞壁的戊聚糖成分限制了葡聚糖的提取。
beta -Glucan can be solubilized from barley by warm water, with increasing solubilization as the temperature is increased. Substantially less glucan is extracted if the barley is dehusked using sulfuric acid, particularly if the dehusked barley is denatured. This indicates that enzymes capable of solubilizing glucan are present in barley. Various purified enzymes promote the solubilization of glucan from denatured and dehusked barley. Apart from endo-beta-(1-->3)(1-->4)-glucanase, these enzymes inelude endo-xylanases, arabinofuranosidase, xyloacetylesterase, and feruloyl esterase. Ferulic acid and, probably, acetyl groups are ester-linked to arabinoxylan, not beta -glucan, in the cell walls of barley starchy endosperm, so the ability of the esterases, xylanases, and arabinofuranosidase to solubilize glucan indicates the pentosan component of the cell wall can restrict the extraction of glucan.