ONTOGENETIC DEVELOPMENT OF SEROTONINERGIC NEURONS IN THE BRAIN OF A TELEOST, THE 3-SPINED STICKLEBACK - AN IMMUNOHISTOCHEMICAL ANALYSIS
ONTOGENETIC DEVELOPMENT OF SEROTONINERGIC NEURONS IN THE BRAIN OF A TELEOST, THE 3-SPINED STICKLEBACK - AN IMMUNOHISTOCHEMICAL ANALYSIS
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DOI：
10.1016/0165-3806(85)90145-2
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
VANVEEN, T
中科院分区：
文献类型：
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作者：
EKSTROM, P;NYBERG, L;VANVEEN, T
用5-羟色胺（5-HT）特异性抗体，用间接免疫细胞化学过氧化物酶-抗过氧化物酶技术，研究了棘鱼脑内5-羟色胺能神经元的个体发育。神经元群体的形成发生在胚胎发育期间。受精后80 h，第一个5-HT胞体出现在下丘脑室区（外侧隐窝核）和中缝区。108 h，在前盖区出现第一个5-HT胞体。在118小时，一个短暂的5-HT神经元群出现在外侧隐窝核的吻侧，第一个5-HT胞体可以在后隐窝核中看到。受精后120-144 h，即孵化后1 d，背外侧被盖区出现一群5-HT神经元。分化的神经元群体，在迁移和形成的细分，开始之间的80小时和94小时，似乎是完成孵化后1至5天。中缝核形成前组包括中缝背核、中缝内侧核和腹外侧组，后组包括中缝苍白暗核复合体、外侧网状旁巨细胞核和中缝大腹内侧核。中缝后核和中缝腹核，这是在孵化时发育良好，在成人stickelback没有可视化。虽然5-HT神经元系统的形成，以及它们的主要传出通路，发生在早期个体发育，终端领域的建立和随后的分化显然发生在后期个体发育阶段。大多数假定的目标区域在孵化时被5-HT轴突穿透，尽管终端形成似乎直到后来才开始。在胚胎和刚孵化的棘鱼中存在相当数量的5-HT神经元群，而在成体棘鱼中则没有。这可能是由于选择性的细胞死亡，递质表型的变化或成熟的轴突运输过程中的发展。
The ontogenetic development of serotoniergic neurons in the brain of the stickleback was investigated with the indirect immunocytochemical peroxidase-antiperoxidase technique, using a specific antibody to serotonin (5-hydroxytryptamine, 5-HT). Formation of neuronal populations takes place during embryonic development. By 80 h after fertilization, the first-5HT perikarya have appeared in the ventricular zone of the hypothalamus (nucleus recessus lateralis) and the raphe region. At 108 h the first 5-HT perikarya can be observed in area praetectalis. At 118 h a transient group of 5-HT neurons appears rostral to the nucleus recessus lateralis and the first 5-HT perikarya may be visualized in nucleus recessus posterioris. A group of 5-HT neurons appears in the dorsolateral tegmentum at 166 h (1 day after hatching, which occurs at 120-144 h after fertilization). Differentiation of the neuronal populations, in terms of migration and formation of subdivisions, starts between 80 h and 94 h and seems to be completed between 1 and 5 days after hatching. Raphe nuclei form an anterior group comprising nuclei raphe dorsalis, raphe medialis and a ventrolateral group, and a posterior group comprising a nucleus raphe pallidus obscurus complex, a lateral nucleus reticularis paragigantocellularis and a ventromedial nucleus raphe magnus. The posterior and ventral raphe nuclei, which are well developed at the time of hatching, were not visualized in the adult stickelback. While formation of 5-HT neuronal systems, as well as their primary efferent pathways, takes place during early ontogenetic development, the establishment of terminal areas and their subsequent differentiation apparently takes place during later ontogenetic stages. Most presumptive target areas are penetrated by 5-HT axons at hatching, although terminal formation does not seem to start until later. A considerable number of 5-HT neuronal groups present in the embryonic and newly hatched stickleback were not visualized in the adult stickleback. This may be due to selective cell death, changes in transmitter phenotype or maturation of axonal transport processes during development.