Human cDNA clones for four species of G alpha s signal transduction protein.
Human cDNA clones for four species of G alpha s signal transduction protein.
复制标题
四种 G α s 信号转导蛋白的人类 cDNA 克隆。
DOI：
--
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
M. Nirenberg
中科院分区：
文献类型：
--
作者：
P. Bray;A. Carter;C. Simons;V. Guo;C. Puckett;J. Kamholz;A. Spiegel;M. Nirenberg
用编码G信号转导蛋白α亚基的重组体的寡核苷酸探针筛选来自人脑的λ gt 11 cDNA文库。用两种探针检测了11个α s克隆并进行了表征。克隆了四种类型的α s cDNA，它们在对应于氨基酸残基71-88的区域中的核苷酸序列不同。克隆的不同之处在于α s氨基酸残基71（谷氨酸或天冬氨酸）的密码子、接下来15个氨基酸残基的密码子的存在或不存在以及相邻丝氨酸残基的存在或不存在。S1核酸酶保护实验揭示了至少两种形式的α s mRNA。提出了前体RNA选择性剪接产生四种α s mRNA的机制。
lambda gt11 cDNA libraries derived from human brain were screened with oligonucleotide probes for recombinants that code for alpha subunits of G signal transduction proteins. Eleven alpha s clones were detected with both probes and characterized. Four types of alpha s cDNA were cloned that differ in nucleotide sequence in the region that corresponds to amino acid residues 71-88. The clones differ in the codon for alpha s amino acid residue 71 (glutamic acid or aspartic acid), the presence or absence of codons for the next 15 amino acid residues, and the presence or absence of an adjacent serine residue. S1 nuclease protection experiments revealed at least two forms of alpha s mRNA. A mechanism for generating four species of alpha s mRNA by alternative splicing of precursor RNA is proposed.