Oxidative stress and airway inflammation after allergen challenge evaluated by exhaled breath condensate analysis
Oxidative stress and airway inflammation after allergen challenge evaluated by exhaled breath condensate analysis
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DOI：
10.1111/j.1365-2222.2010.03604.x
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发表时间：
2010-11-01
影响因子：
6.1
通讯作者：
Rolla, G.
中科院分区：
文献类型：
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作者：
Brussino, L.;Badiu, I.;Rolla, G.
P>背景过敏原暴露可能会增加气道氧化应激，从而导致脂质膜过氧化和8-异前列腺素的形成增加。目的本研究的目的是通过测量呼出气冷凝物（EBC）中的8-异前列腺素，研究过敏原激发引起的轻度哮喘患者的氧化应激，并检查它们与花生四烯酸衍生介质的关系。方法8-异前列腺素、半胱氨酰白三烯对 12 名对猫过敏原敏感的轻度过敏性哮喘患者在基线时和过敏原激发后测量 EBC 中的 (cys-LTs) 和前列腺素 E2 (PGE(2)) 浓度。结果在过敏原激发后 24 小时，与基线值相比，EBC 8-异前列腺素增加 [48.64 pg/mL (44.14-53.61) vs. 21.56 pg/mL] (19.92, 23.35), P < 0.001]，cys-LT 增加 [27.37 pg/mL (24.09-31.10) 对比 13.28 pg/mL (11.32, 15.57)，P < 0.001]，PGE(2) 减少 [18.69 pg/mL (12.26, 28.50) 对比 39.95 pg/mL (34.37, 46.43)，P < 0.001]。过敏原激发后 8-异前列腺素增加的趋势与 cys-LT 增加的趋势显着相关（R2=0.85，P < 0.001），而过敏原激发后 PGE(2) 减少的趋势与 cys-LT 增加的趋势显着相关（R2=0.52，P=0.001）。结论和临床相关性观察后观察到的 EBC 8-异前列腺素增加过敏原挑战表明过敏原暴露会增加过敏性哮喘的气道氧化应激。 cys-LTs 和 8-异前列烷之间的严格相关性强调了过敏性炎症和氧化应激之间的关系。过敏原挑战诱导花生四烯酸代谢向脂氧合酶途径转变。即使在轻度间歇性过敏性哮喘患者中，过敏原激发后也会发生气道氧化应激。引用如下：L. Brussino、I. Badiu S. Sciascia、M. Bugiani、E. Heffler G. Guida、A. Malinovschi、C. Bucca 和 G. Rolla，临床与实验过敏，2010 (40) 1642-1647。
P>BackgroundAllergen exposure may increase airway oxidative stress, which causes lipid membrane peroxidation and an increased formation of 8-isoprostane.ObjectiveThe aim of the study was to investigate oxidative stress induced by allergen challenge in mild asthmatics, by measuring 8-isoprostane in exhaled breath condensate (EBC), and to examine their relationship with mediators derived from arachidonic acid.Methods8-isoprostane, cysteinyl leukotrienes (cys-LTs) and prostaglandin E2 (PGE(2)) concentrations in EBC were measured at baseline and after allergen challenge in 12 patients with mild allergic asthma sensitized to cat allergen.ResultsAt 24 h after allergen challenge, compared with baseline values, EBC 8-isoprostane increased [48.64 pg/mL (44.14-53.61) vs. 21.56 pg/mL (19.92, 23.35), P < 0.001], cys-LTs increased [27.37 pg/mL (24.09-31.10) vs. 13.28 pg/mL (11.32, 15.57), P < 0.001] and PGE(2) decreased [18.69 pg/mL (12.26, 28.50) vs. 39.95 pg/mL (34.37, 46.43), P < 0.001]. The trend of increasing 8-isoprostane after allergen challenge was significantly correlated with the trend of increasing cys-LTs (R2=0.85, P < 0.001) whereas the trend of decreasing PGE(2) after allergen challenge was significantly correlated with the trend of increasing cys-LTs (R2=0.52, P=0.001).Conclusions and Clinical RelevanceThe increase in EBC 8-isoprostane observed after allergen challenge indicates that allergen exposure increases airway oxidative stress in allergic asthma. The strict correlation between cys-LTs and 8-isoprostane underlines the relationship between allergic inflammation and oxidative stress. A shift of arachidonic acid metabolism towards lipoxygenase pathway is induced by the allergen challenge. Airway oxidative stress occurs after allergen challenge even in patients with mild intermittent allergic asthma.Cite this as: L. Brussino, I. Badiu S. Sciascia, M. Bugiani, E. Heffler G. Guida, A. Malinovschi, C. Bucca and G. Rolla, Clinical & Experimental Allergy, 2010 (40) 1642-1647.