EHD2 inhibits ligand-induced endocytosis and signaling of the leucine-rich repeat receptor-like protein LeEix2
EHD2 inhibits ligand-induced endocytosis and signaling of the leucine-rich repeat receptor-like protein LeEix2
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DOI：
10.1111/j.1365-313x.2009.03897.x
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发表时间：
2009-08-01
期刊：
影响因子：
7.2
通讯作者：
Avni, Adi
中科院分区：
文献类型：
--
作者：
Bar, Maya;Avni, Adi
植物不断受到病原体的挑战。为了保护自己，植物已经发展了许多防御机制，这些防御机制对病原体是特异性的或非特异性的。模式识别受体可以触发植物对特定配体或模式的防御反应。EIX（乙烯诱导木聚糖酶）通过LeEix 2受体触发防御反应，而细菌鞭毛蛋白通过FLS 2受体触发植物先天免疫。在这两种情况下，内吞作用都被认为是这一过程的关键。在这里，我们表明，EIX激发子触发LeEix 2受体的内化。内吞作用，肌动蛋白或微管抑制剂的治疗大大降低了LeEix 2的内化。此外，我们证明了植物EHD 2与LeEix 2结合，并且在其内化和调节防御反应的诱导中是重要的因子，所述防御反应例如在EIX/LeEix 2的情况下的过敏反应、乙烯生物合成和致病相关蛋白表达的诱导（缺乏激酶结构域的LRR受体），但似乎不参与FLS 2系统（具有激酶结构域的LRR受体）。我们的研究结果表明，多种内吞途径参与诱导植物防御反应。
Plants are constantly being challenged by aspiring pathogens. In order to protect themselves, plants have developed numerous defense mechanisms that are either specific or non-specific to the pathogen. Pattern recognition receptors can trigger plant defense responses in response to specific ligands or patterns. EIX (ethylene-inducing xylanase) triggers a defense response via the LeEix2 receptor, while bacterial flagellin triggers plant innate immunity via the FLS2 receptor. Endocytosis has been suggested to be crucial for the process in both cases. Here we show that the EIX elicitor triggers internalization of the LeEix2 receptor. Treatment with endocytosis, actin or microtubule inhibitors greatly reduced the internalization of LeEix2. Additionally, we demonstrate that plant EHD2 binds to LeEix2 and is an important factor in its internalization and in regulation of the induction of defense responses such as the hypersensitive response, ethylene biosynthesis and induction of pathogenesis-related protein expression in the case of EIX/LeEix2 (an LRR receptor lacking a kinase domain), but does not appear to be involved in the FLS2 system (an LRR receptor possessing a kinase domain). Our results suggest that various endocytosis pathways are involved in the induction of plant defense responses.