RADIOACTIVE LABELING OF ANTIBODY - A SIMPLE AND EFFICIENT METHOD
RADIOACTIVE LABELING OF ANTIBODY - A SIMPLE AND EFFICIENT METHOD
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DOI：
10.1126/science.6836304
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
DOHERTY, PW
中科院分区：
文献类型：
--
作者：
HNATOWICH, DJ;LAYNE, WW;DOHERTY, PW
由于杂交瘤技术的发展，用放射免疫学方法检测肿瘤组织正受到相当大的重视。一种评估是，使用放射性标记单克隆抗体将消除许多问题，当亲和纯化抗体用于此目的时发生。建立了一种简单有效的强螯合剂二乙烯三胺五乙酸与蛋白质共价偶联的方法，用于IgG抗体的放射性标记。与in -111偶联并标记后，一种针对癌胚抗原的单克隆抗体在体内和体外均保持了与癌胚抗原结合的能力。在移植了人类结直肠癌异种移植物的裸鼠中，注射的放射性在24小时内在每克异种移植物中定位的比例为41%，而对照抗体为9%，癌胚抗原放射性碘化抗体为19%。
The detection of tumor tissue by radioimmunological methods is receiving considerable attention as a result of the development of hybridoma technology. One assessment is that the use of radiolabeled monoclonal antibody will obviate many of the problems that occur when affinity purified antibody is used for this purpose. A simple and efficient method of covalently coupling the strong chelator diethylenetriaminepentaacetic acid to proteins was developed for radiolabeling IgG antibodies. After being coupled and labeled with In-111, a monoclonal antibody to carcinoembryonic antigen retained its ability to bind to its antigen in vitro and in vivo. In nude mice with a human colorectal cancer xenograft, 41% of the injected radioactivity became localized in each gram of xenograft at 24 h compared with 9% for control antibody and 19% for radioiodinated antibody to carcinoembryonic antigen.