Streptococcus pyogenes TrxSR Two-Component System Regulates Biofilm Production in Acidic Environments
Streptococcus pyogenes TrxSR Two-Component System Regulates Biofilm Production in Acidic Environments
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DOI：
10.1128/iai.00360-21
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发表时间：
2021-11-01
影响因子：
3.1
通讯作者：
Hasegawa, Tadao
中科院分区：
文献类型：
--
作者：
Isaka, Masanori;Okamoto, Akira;Hasegawa, Tadao
细菌形成生物膜以保护其免受环境胁迫，并在生物膜内产生毒力因子。在酸化环境中的生物膜的形成是由一个双组分系统，如研究所示的传感器蛋白的同源突变体的双组分调控系统在化脓性链球菌。在这项研究中，我们发现，LiaS组氨酸激酶传感器介导的生物膜的生产和菌毛表达在酸化环境中，通过葡萄糖发酵。liaS同基因突变体在盐酸而不是葡萄糖酸化的培养物中产生生物膜，这表明酸化的环境被另一种蛋白质感知。此外，trxS同基因突变体不能产生生物膜或激活的mga启动子在酸化的环境中。质谱分析表明TrxS对M蛋白的调控与编码M蛋白的emm的转录调控一致。我们的研究结果表明，生物膜生产过程中的环境酸化是直接控制的TrxS。
Bacteria form biofilms for their protection against environmental stress and produce virulence factors within the biofilm. Biofilm formation in acidified environments is regulated by a two-component system, as shown by studies on isogenic mutants of the sensor protein of the two-component regulatory system in Streptococcus pyogenes. In this study, we found that the LiaS histidine kinase sensor mediates biofilm production and pilus expression in an acidified environment through glucose fermentation. The liaS isogenic mutant produced biofilms in a culture acidified by hydrochloric acid but not glucose, suggesting that the acidified environment is sensed by another protein. In addition, the trxS isogenic mutant could not produce biofilms or activate the mga promoter in an acidified environment. Mass spectrometry analysis showed that TrxS regulates M protein, consistent with the transcriptional regulation of emm, which encodes M protein. Our results demonstrate that biofilm production during environmental acidification is directly under the control of TrxS.