Comparison of 3 Techniques of Fat Grafting and Cell-Supplemented Lipotransfer in Athymic Rats: A Pilot Study
Comparison of 3 Techniques of Fat Grafting and Cell-Supplemented Lipotransfer in Athymic Rats: A Pilot Study
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DOI：
10.1177/1090820x13487371
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发表时间：
2013-07-01
影响因子：
2.9
通讯作者：
Elisseeff, Jennifer
中科院分区：
文献类型：
--
作者：
Conde-Green, Alexandra;Wu, Iwen;Elisseeff, Jennifer
背景资料：鉴于自体脂肪移植的广泛应用，一个新的重点脂肪处理技术已经出现，以努力限制不可预测的程度的吸收经常看到这个程序。随着人们对再生医学的兴趣日益浓厚，用脂肪源性干细胞补充脂肪移植物的方法也在不断发展，以期促进血管化和新脂肪形成。目的：作者评价了最常见的脂肪移植处理技术的结果：滗析，洗涤，高速离心和基质血管细胞-补充的脂肪转移，以确定哪种方法产生更高的保留百分比和更好的移植质量。在8只无胸腺大鼠中共进行了32次经处理的人脂肪抽吸物皮下注射。每只动物接受所有4种处理条件，终点为注射后4、8和12周。移植物存活率的评估包括一系列测量的体积保留和组织学analysis.Results：在注射后12周，细胞补充和离心移植物表现出最一致的体积维护。基于组织学分析，细胞补充和洗涤移植物的活力和血管有较高的分数，前者提出了最少的囊性坏死和钙化以及最小的inflammation.Conclusions：细胞补充lipotransfer移植保留，活力和血管的最佳结果，而洗涤导致高活力与较低的密集过程。高速离心导致一致的体积保留，但活力较低。这些方法中的每一种在不同的情况下都是理想的，并有助于脂肪移植的多功能性和可靠性。
Background: Given the wide application of autologous fat grafting, a new emphasis on fat processing techniques has emerged in an effort to limit unpredictable degrees of resorption often seen with this procedure. With the growing interest in regenerative medicine, approaches to supplement fat grafts with adipose-derived stem cells are evolving in hopes of promoting vascularization and neoadipogenesis.Objective: The authors evaluated the outcomes of the most common processing techniques for fat graftingdecantation, washing, high-speed centrifugationand stromal vascular cell-supplemented lipotransfer to determine which method yields a higher percentage of retention and better quality graft.Methods: A total of 32 subcutaneous injections of processed human lipoaspirate were carried out in 8 athymic rats. Each animal received all 4 processing conditions, with end points at 4, 8, and 12 weeks postinjection. Evaluation of graft survival included serial measurements of volume retention and histologic analysis.Results: At 12 weeks postinjection, cell-supplemented and centrifuged grafts showed the most consistent volume maintenance. Based on histologic analysis, cell-supplemented and washed grafts had higher scores of viability and vascularity, with the former presenting the least cystic necrosis and calcification as well as minimal inflammation.Conclusions: Cell-supplemented lipotransfer had optimal outcomes for graft retention, viability, and vascularity, while washing resulted in high viability with a less intensive process. High-speed centrifugation resulted in consistent volume retention but lower viability. Each of these approaches is ideal under different circumstances and contributes to the versatility and reliability of fat grafting.