The role of class I integrons in the dissemination of sulfonamide resistance genes in the Pearl River and Pearl River Estuary, South China
The role of class I integrons in the dissemination of sulfonamide resistance genes in the Pearl River and Pearl River Estuary, South China
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DOI：
10.1016/j.jhazmat.2014.06.010
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发表时间：
2015-01-23
影响因子：
13.6
通讯作者：
Li, Xiangdong
中科院分区：
文献类型：
--
作者：
Chen, Baowei;Liang, Ximei;Li, Xiangdong
抗生素耐药基因（Antibiotic resistance genes, ARGs）作为一种新兴的污染物，其独特之处在于其通过水平基因转移在环境中传播。本研究在珠江口至珠江口的水样和沉积物样品中测定了1类整合子基因（Int1）和多种ARGs (sul1、sul2、sul3、qnrS和ermB)，该区域的人为影响梯度明显。在所有样品中均检测到int1、sul1和sul2基因，其浓度呈明显的下降趋势，与人为影响一致。int1和sul基因都在水与沉积物之间动态迁移。归一化为16S rRNA基因的Intl基因相对丰度与水体和沉积物中抗生素总浓度显著相关。在样品中，Intl的丰度与每种类型的sul基因之间也观察到良好的相关性。然而，sul1基因在不同季节表现出更强的与intl的关系，这可能是由于sul1存在于第1类整合子的保守区域。我们的研究结果强烈支持整合子在人类影响的水生环境中对ARGs的传播起重要作用。(C) 2014 Elsevier B.V.版权所有
Antibiotic resistance genes (ARGs), as a newly emerging contaminant, are unique because they are disseminated through horizontal gene transfer in the environment. In the present study, a class 1 integron gene (Int1) and various ARGs (sul1, sul2, sul3, qnrS, and ermB) were measured in water and sediment samples from the Pearl River (PR) to the Pearl River Estuary (PRE), where there is a distinct gradient in anthropogenic impact. The int1, sul1, and sul2 genes were detected in all samples, and their concentrations exhibited a clear trend of decline consistent with anthropogenic impact. Both the int1 and sul genes had dynamically migrated between water and sediments. The relative abundance of the Intl gene normalized to the 16S rRNA gene correlated significantly with the total concentrations of antibiotics in water and sediments. Good correlations were also observed between the abundance of Intl and each type of sul gene in the samples. However, the sul1 gene showed a much stronger relationship with intl in different seasons, probably due to the presence of sul1 in the conserved region of class 1 integron. Our results strongly support that integrons play an important role in the dissemination of ARGs in human-impacted aquatic environments. (C) 2014 Elsevier B.V. All rights reserved.