Exosomal-like vesicles are present in human blood plasma
Exosomal-like vesicles are present in human blood plasma
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DOI：
10.1093/intimm/dxh267
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发表时间：
2005-07-01
影响因子：
4.4
通讯作者：
Bonnerot, C
中科院分区：
文献类型：
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作者：
Caby, MP;Lankar, D;Bonnerot, C
外泌体是由大多数造血细胞分泌的小膜囊泡（直径50-90 nm）。我们在这里提供了第一个证据外泌体在体内的存在，在血液中。测试的所有健康供体（n = 15）的血浆样品含有在形状、大小和密度上与先前描述的外泌体相似的囊泡。它们被清楚地确定了电子显微镜分离后，通过微分ultracentragation或免疫隔离与CD 63包被的乳胶珠。我们使用一组mAb，通过蛋白质印迹分析和囊泡吸附到乳胶珠上后通过流式细胞术对其进行生化表征。我们观察到，这些血浆衍生的囊泡含有四跨膜蛋白分子，如CD 63、CD 9、CD 81以及I类和II类MHC分子和Lamp-2（即已知在外来体中富集的蛋白质）。此外，这些囊泡以类似于外来体的密度漂浮在蔗糖梯度上。我们的研究结果表明，血液是体内外泌体循环的生理流体，这表明它们在细胞-细胞或器官-器官通信中的作用是作为需要到达远处细胞靶点的分子的载体。
Exosomes are small membrane vesicles (50-90 nm in diameter) secreted by most hematopoietic cells. We provide here the first evidence for the presence of exosomes in vivo, in the blood. Plasma samples of all healthy donors tested (n = 15) contain vesicles that are similar in shape, size and density to the previously described exosomes. They were clearly identified by electron microscopy after isolation by differential ultracentrifugation or immunoisolation with CD63-coated latex beads. We performed their biochemical characterization by western blot analysis and by flow cytometry after vesicle adsorption onto latex beads using a panel of mAbs. We observed that these plasma-derived vesicles contain tetraspanin molecules such as CD63, CD9, CD81 as well as class I and class II MHC molecules and Lamp-2 (i.e. proteins that are known to be enriched in exosomes). In addition, these vesicles float on sucrose gradient at a density similar to exosomes. Our results demonstrate that blood is a physiological fluid for exosome circulation in the body, suggesting their role in cell-cell or organ-organ communications as carriers for molecules that need to reach distant cell targets.