Tobacco Mosaic Virus (TMV) Inhibitors from Picrasma quassioides Benn
Tobacco Mosaic Virus (TMV) Inhibitors from Picrasma quassioides Benn
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DOI：
10.1021/jf901632j
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发表时间：
2009-08-12
影响因子：
6.1
通讯作者：
Hao, Xiao-Jiang
中科院分区：
文献类型：
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作者：
Chen, Jia;Yan, Xiao-Hui;Hao, Xiao-Jiang
为研究植物抗烟草花叶病毒（TMV）的天然抑制剂，从苦木木（Picrasma quassioides Benn.）的甲醇提取物中分离得到10个已知的β-咔啉生物碱和1个苦木素类生物碱。对这些化合物进行了抗烟草花叶病毒（TMV）活性筛选。使用半叶测定法、叶盘法和蛋白质印迹分析来测试每种化合物针对TMV感染和复制的活性。所有的β-咔啉生物碱都显示出中等的抗TMV活性，并且当与苦木素黑杨内酯B组合时显示出协同效应（11）。据我们所知，这是第一份关于β-咔啉类化合物的抗TMV活性及其与苦木素类化合物组合时对TMV的协同作用的报告。
To investigate natural inhibitors against tobacco mosaic virus (TMV) from plants, 10 known beta-carboline alkaloids and one quassinoid have been isolated from MeOH extract of the wood of Picrasma quassioides Benn. These compounds were screened for their inhibitory activities against tobacco mosaic virus (TMV). The activity of each compound against TMV infection and replication was tested using a half-leaf assay method, a leaf-disk method, and Western blotting analyses. All of the beta-carboline alkaloids showed moderate anti-TMV activities and exhibited synergistic effects when combined with the quassinoid nigakilactone B (11). To our knowledge, this is the first report on anti-TMV activity of beta-carbolines and their synergistic effects against TMV when combined with a quassinoid.