GENETIC-ANALYSIS OF A LARGE AUTOSOMAL REGION IN CAENORHABDITIS-ELEGANS BY THE USE OF A FREE DUPLICATION
GENETIC-ANALYSIS OF A LARGE AUTOSOMAL REGION IN CAENORHABDITIS-ELEGANS BY THE USE OF A FREE DUPLICATION
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DOI：
10.1017/s0016672300027099
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发表时间：
1987-06-01
影响因子：
1.5
通讯作者：
ROSE, AM
中科院分区：
文献类型：
--
作者：
HOWELL, AM;GILMOUR, SG;ROSE, AM
在本文中，我们描述了一种自由重复序列sDp2（I；f）在秀丽隐杆线虫连锁群I左三分之一区域中对定义必需基因的突变进行恢复、维持和分析的用途。这些致死突变是在基因型为（sDp2） + /dpy - 5 + unc - 13/dpy - 5 unc - 15 +的菌株中诱导产生的，使用的诱变剂为12 mM的乙基甲磺酸酯或1500伦琴的伽马辐射。与dpy - 5 - unc - 13染色体连锁的致死突变通过在自交后代中没有Dpy - 5 Unc - 13个体而被识别出来，并通过分离Unc - 13雌雄同体来维持。这些菌株——在重复序列上有必需基因的两个突变等位基因和一个野生型等位基因——是平衡的，因为sDp2和正常同源染色体之间不会发生交换。利用这个系统，我们已经恢复了58个由EMS诱导的突变。这些突变在图谱位置和互补性方面已经得到了鉴定。29个由EMS诱导的突变位于dpy - 5左侧，定义了20个互补群；3个与dpy - 5无法区分，定义了3个互补群；21个位于右侧，定义了17个互补群。在一组29个由伽马辐射诱导的致死突变中，17个似乎是单基因突变或非常小的缺失。我们估计我们已经鉴定出了sDp2区域中六分之一到二分之一的必需基因。
In this paper we describe the use of a free duplication, sDp2 (I;f), for the recovery, maintenance and analysis of mutations defining essential genes in the left third of Linkage Group I of Caenorhabditis elegans. The lethals were induced in a strain of genotype (sDp2) + /dpy-5 + unc-13/dpy-5 unc-15+, using either 12 mM ethylmethane sulphonate or 1500 r of gamma radiation. Lethal mutations linked to the dpy-5-unc-13 chromosome were recognized by the absence of Dpy-5 Unc-13 individuals amongst the self progeny and were maintained by isolating Unc-13 hermaphrodites. These strains-which have two mutant alleles of the essential gene and a wild-type allele on the duplication-are balanced, since crossing-over does not occur between sDp2 and the normal homologues. Using this system we have recovered 58 EMS-induced mutations. These have been characterized with regard to map position and complementation. Twenty-nine of the EMS-induced mutations lie to the left of dpy-5 and define 20 complementation groups; 3 were inseparable from dpy-5 and define 3 complementation groups; 21 were to the right and define 17 complementation groups. Among a set of 29 gamma radiation-induced lethal mutations, 17 appear to be single gene mutations or are very small deletions. We estimate that we have identified from one-sixth to one-half of the essential genes in the sDp2 region.