Fetal cortical transplants in adult rats subjected to experimental brain injury.
Fetal cortical transplants in adult rats subjected to experimental brain injury.
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遭受实验性脑损伤的成年大鼠的胎儿皮质移植。
DOI：
10.1155/np.1991.207
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
McIntosh,TK
中科院分区：
文献类型：
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作者：
Soares,H;McIntosh,TK
将胎儿皮质组织注射到震荡性液压冲击（FP）脑损伤后的成年大鼠脑内。受到中度FP损伤的大鼠在损伤后2天、1周、2周和4周接受E16皮质移植注射到受损的运动皮质中。移植物存活和整合的组织学评估基于尼氏染色、胶质细胞酸性蛋白（GFAP）免疫细胞化学和乙酰胆碱酯酶染色。除了组织学分析之外，还测试了移植物减轻与震荡性FP脑损伤相关的神经运动缺陷的能力。选择伤后1周、2周和4周接受移植的三个亚组的大鼠进行神经运动功能评价。损伤后4周，胎儿皮质组织注射到损伤部位未能与受损的宿主脑结合，不影响胶质瘢痕形成，并表现出广泛的GFAP免疫反应性。在损伤后4周接受移植的动物中没有观察到神经运动功能的改善。相反，损伤后2天、1周或2周注射的移植物存活，与宿主脑结合，表现出很少的GFAP免疫反应性，并成功地减弱了胶质瘢痕形成。然而，在一周或两周时间点未观察到运动功能的显著改善。移植物不能减弱运动功能可能表明不适当的宿主/移植物相互作用。我们的研究结果表明，存在一个时间窗口，胎儿皮质移植可以减轻胶质瘢痕形成，以及成功地纳入宿主脑后FP损伤。
Fetal cortical tissue was injected into injured adult rat brains following concussive fluid percussion (FP) brain injury. Rats subjected to moderate FP injury received E16 cortex transplant injections into lesioned motor cortex 2 days, 1 week, 2 weeks, and 4 weeks post injury. Histological assessment of transplant survival and integration was based upon Nissl staining, glial fibrillary acidic protein (GFAP) immunocytochemistry, and staining for acetylcholinesterase. In addition to histological analysis, the ability of the transplants to attenuate neurological motor deficits associated with concussive FP brain injury was also tested. Three subgroups of rats receiving transplant 1 week, 2 weeks, and 4 weeks post injury Were chosen for evaluation of neurological motor function. Fetal cortical tissue injected into the injury site 4 weeks post injury failed to incorporate with injured host brain, did not affect glial scar formation, and exhibited extensive GFAP immunoreactivity. No improvement in neurological motor function was observed in animals receiving transplants 4 weeks post injury. Conversely, transplants injected 2 days, 1 week, or 2 weeks post injury survived, incorporated with host brain, exhibited little GFAP immunoreactivity, and successfully attenuated glial scarring. However, no significant improvement in motor function was observed at the one week or two week time points. The inability of the transplants to attenuate motor function may indicate inappropriate host/transplant interaction. Our results demonstrate that there exists a temporal window in which fetal cortical transplants can attenuate glial scarring as well as be successfully incorporated into host brains following FP injury.