The chemical and digestive properties of a soluble glucan from Agrobacterium sp. ZX09
The chemical and digestive properties of a soluble glucan from Agrobacterium sp. ZX09
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DOI：
10.1016/j.carbpol.2010.05.027
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发表时间：
2010-10
影响因子：
11.2
通讯作者：
Aihui Xiu;Yi Kong;Mengyi Zhou;Bin Zhu;Shiming Wang;Jianfa Zhang
中科院分区：
文献类型：
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作者：
Aihui Xiu;Yi Kong;Mengyi Zhou;Bin Zhu;Shiming Wang;Jianfa Zhang
耐盐菌株Agrobacterium sp. ZX 09产生一种高分子量的水溶性胞外多糖（EPS），该多糖由d-葡萄糖组成。通过高碘酸氧化、1H NMR和13 C NMR谱的研究，证实EPS由以下重复单元组成：→3）-β-d-Glcp-（1→3）-[β-d-Glcp-（1 →3）-β-d-Glcp-（1→3）]3-α-d-Glcp-（1→3）-α-d-Glcp-（1→ 3）。EPS在体外不能被α-淀粉酶消化，并强烈抑制胰腺α-淀粉酶活性。以EPS喂养的禁食小鼠未能增加血糖水平。含有EPS的实验饮食抑制血糖浓度的急剧增加。这些结果表明，新型水溶性葡聚糖可能对降低膳食碳水化合物的吸收和餐后血糖水平具有潜在的用途。
A salt-tolerant strain Agrobacterium sp. ZX09 produced a high molecular mass, water-soluble extracellular polysaccharide (EPS) composed of d-glucose. By examining periodate oxidation,1H NMR and13C NMR spectra, it was proven that the EPS consisted of the following repeating unit: →3)-β-d-Glcp-(1→3)-[β-d-Glcp-(1→3)-β-d-Glcp-(1→3)]3-α-d-Glcp-(1→3)-α-d-Glcp-(1→. In vitro the EPS was indigestible by α-amylase, and strongly inhibited pancreatic α-amylase activity. Fasting mice fed on the EPS failed to increase blood glucose levels. Experimental diets containing the EPS suppressed steep increase in blood glucose concentration. These results suggest that the novel water-soluble glucan could be potentially useful for decreasing absorption of dietary carbohydrate and postprandial blood glucose level.