MicroRNA profiling during germline differentiation of mouse embryonic stem cells
MicroRNA profiling during germline differentiation of mouse embryonic stem cells
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DOI：
10.14715/cmb/2015.61.3.16
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发表时间：
2015-01-01
影响因子：
1.6
通讯作者：
Modarressi, M. H.
中科院分区：
文献类型：
--
作者：
Ebrahimzadeh-Vesal, R.;Shokrgozar, M. A.;Modarressi, M. H.
MicroRNAs是一类新的非编码的小分子调控RNA，通过序列互补控制其靶标mRNAs的降解或翻译。成熟的microRNAs富含于胚胎干细胞中，在控制干细胞自我更新和调控分化方面发挥着重要作用。有重要证据表明，microRNAs参与了干细胞分化的调节。以核型正常的雄性小鼠胚胎干细胞系C57BL6/J为材料，对未分化的小鼠胚胎干细胞和分化为生殖系的小鼠胚胎干细胞进行microRNA表达谱分析，以确定并比较分化前后microRNA表达的差异。同时，取一只5日龄小鼠和一只成年小鼠的睾丸组织样本作为体内对照。使用锁定的核酸microRNA特异性LNA(TM)增强的引物，通过实时定量聚合酶链式反应进行分析。经过数据分析和结果比较，MMU-miR-21、MMU-miR-21和MMU-miR-16三个microRNAs在分化的mESCs中的表达分别是未分化状态的50.31、43.76和46.77倍，差异有统计学意义(P<0.05)。
MicroRNAs are new classes of small non-coding regulatory RNAs which control degradation or suppress translation of its target mRNAs by sequence complementarity. Mature microRNAs are enriched in embryonic stem cells and play important roles in controlling stem cell self-renewal as well as control of differentiation. There is significant evidence that microRNAs are involved in the regulation of stem cell differentiation. The male mouse Embryonic Stem Cell line C57BL6/J with normal karyotype 46, XY was used for profiling microRNA expression in undifferentiated mouse embryonic stem cells (mESCs) and mESCs which were differentiated to germ line cells to determine and compare differences in microRNA expression before and after differentiation. Also, testis tissue samples of a 5-day-old mouse and a mature mouse was used as in vivo control. Profiling was performed by quantitative real-time PCR using locked nucleic acid microRNA-specific LNA (TM)-enhanced primers. After data analysis and comparison of results profiled microRNAs expression, three microRNAs, mmu-miR-21, mmu-miR-21* and mmu-miR-16 showed 50.31, 43.76 and 46.77-fold change increase of expression, respectively, in differentiated mESCs in comparison with undifferentiated state with significant p-value (Average p-value p