INFLUENCE OF PHOSPHATE AND OTHER NUTRIENTS ON DEVELOPMENT OF VESICULAR-ARBUSCULAR MYCORRHIZA IN CULTURE
INFLUENCE OF PHOSPHATE AND OTHER NUTRIENTS ON DEVELOPMENT OF VESICULAR-ARBUSCULAR MYCORRHIZA IN CULTURE
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DOI：
10.1099/00221287-69-2-157
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
PHILLIPS, JM
中科院分区：
文献类型：
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作者：
MOSSE, B;PHILLIPS, JM
通过内生孢子接种，在小花车轴草（Trifolium parviflorum）培养基上建立了泡囊-丛枝菌根。在含265 mg.磷（P）/升，CaHPO_4和K_2HPO_4只在缺氮时才能侵染。在培养基中只含有100毫克。P/L.，0·5g.磷酸二氢钙、植酸钙、植酸钠、植酸铁、菲汀、卵磷脂和DNA是植物和真菌的充足磷源。在琼脂培养基中，植酸钙和DNA极大地促进了真菌的生长，DNA也促进了孢子的形成。在培养基中加入Na后，根中的感染很少。肌醇可以作为一个碳源Endogone.Mycorrhizal感染发生与FeCl 3或Fe-EDTA在培养基中，当铁是如此之少，植物生长不良，也有很少的菌根感染。
Vesicular-arbuscular mycorrhiza were established inTrifolium parviflorumin culture by inoculation with Endogone spores. In a medium containing 265 mg. phosphorus (P)/l., as CaHPO4and K2HPO4, infection occurred only when the medium lacked nitrogen (N). In a medium containing only 100 mg. P/l., infection occurred readily in the presence of 0·5 g. KNO3/l.Calcium monohydrogen phosphate, Ca phytate, Na phytate, Fe phytate, phytin, lecithin and DNA were adequate sources of phosphate for both plant and fungus. Ca phytate and DNA greatly stimulated fungal growth, and DNA also stimulated spore formation, in the agar medium. With Na in the medium infections in the root were sparse. Inositol may serve as a carbon source for Endogone.Mycorrhizal infection occurred with either FeCl3or Fe-EDTA in the medium; when so little iron was present that plants grew poorly, there was also little mycorrhizal infection.