Short-chain phosphatidylethanolamines: physical properties and susceptibility of the monomers to phospholipase A2 action.
Short-chain phosphatidylethanolamines: physical properties and susceptibility of the monomers to phospholipase A2 action.
复制标题
短链磷脂酰乙醇胺：单体对磷脂酶 A2 作用的物理性质和敏感性。
作者：
A. Plückthun;R. Rohlfs;F. Davidson;E. Dennis
合成了从二丁酰-PE到二辛酰-PE的光学活性短链磷脂酰乙醇胺（PE）同系物。此外，合成了两种不被磷脂酶A2降解的单体短链磷脂类似物（1，2-双[（丁基氨基甲酰基）氧基]-sn-甘油基-3-磷酸胆碱和相应的乙醇胺衍生物）。与短链磷脂酰胆碱（PC）相反，短链PE在水中具有确定的溶解度。在表面张力图中没有发现低于溶解度极限的突破，表明这些化合物在水溶液中以单体形式存在。只有当大部分分子带负电荷时，它们才能自己形成胶束。眼镜蛇毒磷脂酶A2以与短链PC大致相同的速率水解单体短链PE，但水解长链PC比水解长链PE快得多。发现短链PE的水解仅在界面的存在下被含磷酸胆碱的化合物激活;在其不存在的情况下，含磷酸胆碱的化合物可以作为竞争性抑制剂。这种现象的可能解释被认为是。
The homologous series of optically active short-chain phosphatidylethanolamines (PE) from dibutyryl-PE to dioctanoyl-PE was synthesized. In addition, two monomeric short-chain phospholipid analogues that are not degraded by phospholipase A2 (1,2-bis[(butylcarbamyl)oxy]-sn-glycero-3-phosphocholine and the corresponding ethanolamine derivative) were synthesized. In contrast to the short-chain phosphatidylcholines (PC), short-chain PE's have defined solubilities in water. No break below the solubility limit was found in surface tension plots, suggesting that these compounds exist as monomers in aqueous solution. Only when a significant fraction of the molecules is negatively charged can they form micelles by themselves. Cobra venom phospholipase A2 hydrolyzes monomeric short-chain PE's at about the same rate as short-chain PC's but hydrolyzes long-chain PC's much more rapidly than long-chain PE's. The hydrolysis of short-chain PE's is found to be activated by phosphocholine-containing compounds only in the presence of an interface; in its absence phosphocholine-containing compounds can act as competitive inhibitors. Possible explanations for this phenomenon are considered.
DOI：
10.1016/s0021-9258(18)32418-9
发表时间：
1983-05
期刊：
The Journal of biological chemistry
影响因子：
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作者：
A. Roda;A. Hofmann;K. J. Mysels
通讯作者：
A. Roda;A. Hofmann;K. J. Mysels