Microbial characteristics of dental caries in HIV positive individuals.
Microbial characteristics of dental caries in HIV positive individuals.
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DOI：
10.3389/froh.2022.1004930
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发表时间：
2022
影响因子：
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通讯作者：
Muwonge, Adrian
中科院分区：
文献类型：
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作者：
Kalanzi, Dunstan;Mayanja-Kizza, Harriet;Nakanjako, Damalie;Semitala, Fred;Mboowa, Gerald;Mbabali, Muhammad;Kigozi, Edgar;Katabazi, Fred Ashaba;Sserwadda, Ivan;Kateete, David P;Achan, Beatrice;Sewankambo, Nelson K;Muwonge, Adrian
龋齿是一种影响许多人的多因素疾病。尽管微生物在引起龋齿方面起着至关重要的作用，但诊断通常是宏观的。为了提高早期发现，特别是在艾滋病毒患者谁是不成比例的影响，有必要调和宏观和微观特征的龋齿。因此，本研究的目的是使用扩增子测序数据来表征口腔微生物群分布沿着龋齿、缺失、填充牙齿（DMFT）指数。对从59名HIV阳性和29名HIV阴性个体的完整未刺激唾液中回收的DNA进行16 S rRNA基因的V6-V8区域的扩增子测序。利用QIIME-2、Phyloseq、Microbiome-1.9.2和Metacoder等软件对该菌的结构、组成和共生网络进行了表征。我们从260万个高质量序列读取中将口腔微生物群表征为2，093个操作分类单位（OTU），21个门和239个属。虽然口腔微生物群没有将参与者聚类到跟踪DMFT指数的不同组中，但我们观察到以下情况：（a）在高DMFT类别中，附属微生物群的比例最高，而核心大小（丰富度的约50%）在所有类别中保持相对稳定。(b)核心属如口腔杆菌属、消化链球菌属和弯曲杆菌属的丰度在龋齿开始时是高的。（c）口腔微生物生物量的一般差异。(d)龋齿的发生（低DMFT）与口腔微生物熵显着降低。虽然口腔微生物的变化沿着DMFT指数是不明显的，我们证明了潜在的效用，微生物群动态的特点口腔疾病。因此，我们提出了一个微生物的框架，使用DMFT指数，以更好地了解龋齿之间的艾滋病毒阳性的人在资源有限的设置。
Dental caries is a multifactorial disease that affects many people. Even though microorganisms play a crucial role in causing dental caries, diagnosis is routinely macroscopic. In order to improve early detection especially in HIV patients who are disproportionately affected, there is need to reconcile the macroscopic and microscopic characteristics of dental caries. Therefore, the aim of this study was to characterize the oral microbiota profile along the decayed, missing, filled teeth (DMFT) index using amplicon sequencing data. Amplicon sequencing of the V6-V8 region of the 16S rRNA gene was done on DNA recovered from whole unstimulated saliva of 59 HIV positive and 29 HIV negative individuals. The microbial structure, composition and co-occurrence networks were characterized using QIIME-2, Phyloseq, Microbiome-1.9.2 and Metacoder in R. We characterized the oral microbiota into 2,093 operational taxonomic units (OTUs), 21 phyla and 239 genera from 2.6 million high quality sequence reads. While oral microbiota did not cluster participants into distinct groups that track with the DMFT index, we observed the following: (a) The proportion of accessory microbiota was highest in the high DMFT category while the core size (∼50% of richness) remained relatively stable across all categories. (b) The abundance of core genera such as Stomatobaculum, Peptostreptococcus and Campylobacter was high at onset of dental caries, (c) A general difference in oral microbial biomass. (d) The onset of dental caries (low DMFT) was associated with significantly lower oral microbial entropy. Although oral microbial shifts along the DMFT index were not distinct, we demonstrated the potential utility of microbiota dynamics to characterize oral disease. Therefore, we propose a microbial framework using the DMFT index to better understand dental caries among HIV positive people in resource limited settings.