Pyruvate kinase M2 promotes the growth of gastric cancer cells via regulation of Bcl-xL expression at transcriptional level
Pyruvate kinase M2 promotes the growth of gastric cancer cells via regulation of Bcl-xL expression at transcriptional level
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DOI：
10.1016/j.bbrc.2012.05.063
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发表时间：
2012-06-22
影响因子：
3.1
通讯作者：
Kim, Yong Sung
中科院分区：
文献类型：
--
作者：
Kwon, Oh-Hyung;Kang, Tae-Wook;Kim, Yong Sung
PKM2是促进有氧糖酵解的糖酵解酶丙酮酸激酶的同工酶。在这里，我们描述了PKM2在调节胃癌(GC)细胞生存中的重要作用。我们发现，与正常组织相比，PKM2在胃肿瘤组织中过表达，其表达水平与胃癌患者的生存不良有关。我们还发现，PKM2通过在转录水平上调节Bclxl而影响细胞存活。PKM2基因敲除部分影响了核因子-kB亚单位p65的稳定性，提示PKM2对p65的翻译后调控是促进细胞生长的可能机制之一。因此，PKM2可能是调节p65功能的上游分子，从而促进肿瘤细胞的生长。(C)2012 Elsevier Inc.保留所有权利。
PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase that promotes aerobic glycolysis. Here, we describe an important role for PKM2 in regulating the survival of gastric cancer (GC) cells. We showed that PKM2 was overexpressed in gastric tumor tissues compared to normal tissues and its expression level was associated with poor survival of gastric cancer patients. We also showed that PKM2 affected cell survival by regulating Bcl-xL at the transcriptional level. PKM2 knockdown partially affected the stability of NF-kB subunit p65, suggesting that post-translational regulation of p65 by PKM2 is one of plausible mechanisms for the increased cell growth. Therefore, PKM2 may function as an upstream molecule that regulates p65 function and thus enhances the growth of tumor cells. (C) 2012 Elsevier Inc. All rights reserved.