Effect of different collagenases on the isolation of viable hepatocytes from rat liver.
Effect of different collagenases on the isolation of viable hepatocytes from rat liver.
复制标题
不同胶原酶对从大鼠肝脏中分离活肝细胞的影响。
DOI：
10.1016/0003-2697(84)90794-2
复制
发表时间：
1984
影响因子：
2.9
通讯作者：
Garrett,CT
中科院分区：
文献类型：
--
作者：
Queral,AE;DeAngelo,AB;Garrett,CT
从大鼠肝脏中分离出存活的肝细胞对胶原酶的来源(EC 3.4.24.3)的依赖程度超过了所考察的任何单一因素。从嗜酸杆菌/多粘芽孢杆菌(胶原酶/多粘芽孢杆菌)中纯化的胶原酶在不使用复杂的灌流方案的情况下，具有很高的重复性。
The isolation of viable hepatocytes from rat liver was found to depend on the source of collagenase (EC 3.4.24.3) more than any other single factor examined. Collagenase purified from Achromobacter iophagus/Bacillus polymyxa (collagenase/dispase) gave reproducibly high viability without the use of complex perfusion protocols.