The co-induced effects of molybdenum and cadmium on the mRNA expression of inflammatory cytokines and trace element contents in duck kidneys.
The co-induced effects of molybdenum and cadmium on the mRNA expression of inflammatory cytokines and trace element contents in duck kidneys.
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DOI：
10.1016/j.ecoenv.2016.07.007
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发表时间：
2016-11
影响因子：
6.8
通讯作者：
H. Cao;Feiyan Gao;B. Xia;Qingyang Xiao;Xiaoquan Guo;G. Hu;Caiying Zhang
中科院分区：
文献类型：
--
作者：
H. Cao;Feiyan Gao;B. Xia;Qingyang Xiao;Xiaoquan Guo;G. Hu;Caiying Zhang
本研究旨在探讨饲粮镉（Cd）和高钼（Mo）对鸭肾毒性的联合诱导效应。选用11日龄健康肉鸭240只，随机分为6组，在基础日粮中添加不同剂量的钼或/和镉，试验期120 d。对照组饲喂基础日粮，低钼组和高钼组分别饲喂15 mg/kg和100 mg/kg钼，镉组在基础日粮中添加4 mg/kg镉。两个组合组分别用15 mg/kg Mo+4 mg/kg Cd和100 mg/kg Mo+4 mg/kg Cd处理。分别于第30、60、90、120天检测各组大鼠炎性细胞因子mRNA表达水平和微量元素含量。此外，透射电子显微镜检查用于超微结构研究。结果表明：与对照组相比，各处理组肿瘤坏死因子-α（TNF-α）、核因子-κ B（NF-κB）和环氧合酶-2（考克斯-2）的mRNA表达水平均呈上升趋势，且在实验后期，联合处理组与钼、镉处理组相比均有显著性升高结果表明：联合组大鼠血清中铜、铁含量随时间延长而降低（P <0. 05），钼、镉含量随时间延长而升高（P<0. 01），锌、硒含量随时间延长呈下降趋势，但差异不显著（P> 0. 05）。超微结构分析显示，120天联合组肾组织损伤严重。提示钼、镉联合染毒可加重肾脏的损伤。此外，饲粮中添加Mo或/和Cd可引起Cu、Fe、Zn、Se含量降低、炎症反应和病理损伤，其机制可能与Mo和Cd在肾脏的沉积有关。
The aims of this study were determining the co-induced effects of dietary Cadmium (Cd) and high intake of Molybdenum (Mo) on renal toxicity in ducks. 240 healthy 11-day-old ducks were randomly divided into 6 groups, which were treated with Mo or/and Cd at different doses added to the basal diet for 120 days. Ducks of control group were fed with basal diet, LMo and HMo groups were fed with 15 mg/kg Mo and 100 mg/kg Mo respectively; ducks of Cd group were provided with 4 mg/kg Cd which was added into basal diet. Two combination groups were treated with 15 mg/kg Mo+4 mg/kg Cd and 100 mg/kg Mo+4 mg/kg Cd respectively. On days 30, 60, 90 and 120, the mRNA expression levels of inflammatory cytokines and contents of trace elements were detected. In addition, transmission electron microscopic examination was used for ultrastructural studies. The results indicated that the mRNA expression levels of tumor necrosis factor-α (TNF-α), nuclear factor-kappa B (NF-κB), and cyclooxygenase-2 (COX-2) showed an upward tendency in treatment groups in comparison with control group, and in the later period of the experiment it showed a significant rise in joint groups compared with the Mo and Cd group (P<0.01); the contents of copper (Cu) and iron (Fe) decreased in joint groups in the later period (P<0.05) while the contents of Mo and Cd significantly increased (P<0.01); zinc (Zn) and selenium (Se) concentration had a slight downtrend in treated groups, but showed no significant difference (P>0.05). The ultrastructural analysis showed that kidney tissues were severely injured in joint groups on day 120. These results suggested that the combination of Mo and Cd could aggravate damages to the kidney. In addition, dietary of Mo or/and Cd caused the decrease of Cu, Fe, Zn, and Se contents, inflammatory response and pathological lesions whose mechanism is somehow linked with Mo and Cd deposition in kidney.